N-(O,O-二异丙基)磷酰化丝氨酸成肽反应的立体效应研究

Stereo-effect of the Peptide Formation Reaction by N-(O,O-Diisopropyl) Phosphoryl Serine

研究了 N-( O,O-二异丙基 )磷酰化 -L-(或 D-)丝氨酸和 L-(或 D-)组氨酸在水溶液中的反应及其与 L-(或 D-)组氨酸甲酯在 DMSO中的反应 .用 SDS作对离子试剂 ,采用离子对反相高效液相色谱方法对反应产物进行分析 .结果表明 ,所有反应体系中均有丝组二肽生成 ,而 L-L和 D-D型丝组二肽的生成量约是 L-D和 D-L型产物的

The reactions between N phosphoryl L (or D ) serine and L (or D ) histidine or L (or D ) histidine methyl ester were investigated. Products of the reaction mixtures were analyzed by ion pair reversed phase HPLC with SDS as the ion pairing reagent. The results showed that dipeptide serylhistidine was formed in all these reaction systems, and L L and D D types of serylhistidine were yielded in an amount about four times as much as that of the L D and D L type dipeptide.