三种抑菌剂对蛋白核小球藻生物量和脂质含量的影响

Effect of three bacteriostats on biomass and lipid content of chlorellapyrenoidosa

为寻找能够抑制或消灭产油微藻非共生菌且对产油微藻培养有促进作用的抑菌剂,推动富油微藻生产生物柴油的进程,研究了孟加拉红、硫酸链霉素和制霉菌素3种抑菌剂单独和组合分别对蛋白核小球藻生长的影响,并利用超临界二氧化碳萃取法和索氏提取法测定了藻类细胞中的脂质含量的变化.结果表明,制霉菌素会抑制蛋白核小球藻的生长;孟加拉红和硫酸链霉素对蛋白核小球藻的生长表现出积极的促进作用,藻细胞密度的最大值分别为4.216×107 cells/mL和3.512×107 cells/mL,高于不加任何抑菌剂的对照组3.325×107 cells/mL,而对油脂含量几乎没有影响.孟加拉红和硫酸链霉素的混合抑菌剂对蛋白核小球藻的生长起抑制作用.

To find antibacterial agents for suppression or elimination of non-symbiotic bacteria and promotion of oil-rich microalgae cultivation,and then promote biodiesel production process from microalgae,the effects of rose bengal,nystatin and streptomycin sulphate on the growth of chlorella pyrenoidosa were investigated.The lipid contents in algae cells were measured using supercritical CO2 extraction and soxhlet extraction.The results indicate that rose bengal and streptomycin sulphate have almost no effect on lipid content; however,they have a positive effect on stimulating the growth of Chlorella pyrenoidosa,and the maximum values of algae density are 4.216 × 107 cell/mL and 3.512× 107 cell/mL respectively,which are higher than that of control group （3.325 × 107 cell/mL）without antibiotic.The mixtures of rose bengal and streptomycin sulphate show negative effects on microalgae growth.