木聚糖酶生产菌黑曲霉的诱变及周期变压发酵

Mutation of aspergillus niger producing xylanase and its periodic pressure fermentation

木聚糖酶产生菌黑曲霉An 1经紫外线和硫酸二乙酯 (DES)处理后 ,采用显色平皿法及液体培养筛选 ,得到一产木聚糖酶活力较高的菌株An 1 15 ,其产酶能力比An 1提高 90 %。同时研究了周期变压操作对固态发酵过程中黑曲霉产酶的影响。当变压范围为 0～ 0 15MPa,选取适当变压周期时 ,黑曲霉木聚糖酶酶活以干料计高达 36 90IU·g-1,比静止固态发酵高 38%。

A mutant strain An-1.15 was obtained with the screening of a color plate method and liquid state fermentation after Aspergillus niger An-1 producing xylanase was treated with UV and DES. The xylanase of An-1.15 increased by 90%. The influence of periodic air pressure oscillation on xylanase activities was investigated during the course of solid state fermentation of An-1.15. With the air pressure amplitude of 0-0.15 MPa and proper oscillation period, the xylanase activity was as high as 3,690 IU/g dry medium, which was 38% more than xylanase activity of static solid state fermentation.