中性粒细胞CD64表达在新生儿败血症中的临床意义

Clinical meaning of neutrophil CD64 expression in septicemia of newborn

目的观察外周血中性粒细胞CD64的表达水平,探讨其在新生儿败血症诊断中的临床意义。方法选择2012年1月至2013年2月在新生儿重症监护室(NICU)住院的79例新生儿,根据患儿的高危因素、住院期间的临床症状、体征及实验室检测分为新生儿败血症组(25例)、一般感染组(26例)及非感染组(28例)。应用流式细胞仪测定外周血中性粒细胞CD64表达,并探讨其意义。结果败血症组治疗前外周血中性粒细胞CD64表达高于一般感染组及非感染组(P<0.05,P<0.01);治疗7 d后,败血症组及一般感染组中性粒细胞CD64表达均显著低于治疗前(P均<0.05),但败血症组中性粒细胞CD64表达仍然高于一般感染组及非感染组(P均<0.05)。结论外周血中性粒细胞CD64表达水平可以作为早期诊断新生儿败血症的理想指标,为可疑败血症的高危儿提供实验依据。

Objective To investigate the clinical meaning of CD64 expression on neutrophil of peripheral blood in the newborns with septicemia. Methods Seventy-nine newborns admitted in newborn intensive care unit （NICU） between January 2012 and February 2013 were enrolled in this study. Based on the high risk factors, clini- cal symptoms and signs and laboratory examination, the sick newborns were divided into three groups： septicemia group （25 cases）, common infection group （26 cases） and non-infection group （28 cases）. The neutrophil CD64 expression in peripheral blood was detected by flow cytometry, and its clinical significance was explored. Results The neutrophil CD64 expression in peripheral blood before treatment in septicemia group was significantly higher than those in common infection group and non-infection group （ P 〈 0.05 or P 〈 0.01 ）. Compared with pre-treat- ment, neutrophil CD64 expressions 7 days after treatment in septicemia group and common infection group decreased significantly （ all P 〈 0.05 ）, but the expression in septicemia group remained higher than those in common infection group and non-infection group （ all P 〈 0.05）. Conclusions The expression level of neutrophil CD64 in peripheral blood can be as served a perfect indicator for early diagnosis of newborn septicemia and provide a basis of laboratory examination for the hizh risk newborns with suspicious septicemia.