糖蛋白抗原抗体蚕丝丝素膜免疫传感器

A Silk Fibroin Potentiometric Immunosensor Immobilizing CA15-3 Antibody

蚕丝丝素膜由具有两性离子交换基的聚氨基酸构成 ,是一种具有广泛应用前景的天然生物材料。该文研究了蚕丝丝素膜的制备、蚕丝丝素膜固定乳腺癌抗原CA15 -3抗体的各种方法及原理 ,以及用于CA15 -3非标记免疫传感器的结果及比较。在各种固定方法中 ,以盐酸活化法、叠氮法、重氮法的效果较好。作者用盐酸活化法制作抗体膜并组装免疫电极 ,制作了测定CA15 -3的标准曲线 ,讨论了重复性、干扰情况以及传感器的响应机理。

A silk fibroin membrane consists of polyamino acid with amphoteric ion _ exchange groups. The preparation of silk fibroin and different ways of immobilizing antibody on the fibroin membrane were investigated for preparing an electrochemical immunosensor to determine the cancer marker CA15-3. Adsorption, direct cross _ linking, hydroxylamine activation, hydrochloric acid activation, diazonium and azidonium method were tested to immobilize the antibody. When the silk fibroin was activated with hydrochloric acid, the membrane showed the best potential response. A potentiometric immunosensor was made of an immobilizing antibody membrane and an inner Ag/AgCl reference electrode. There was a linear response of potential over the range of 15～240 ku/L for CA15-3 with a correlation coefficient of 0.999 6. The mechanism of potential response has been discussed.