分子印迹固相萃取-液相色谱联用法测定3种新烟碱类农药的残留

Determination of Three Neonicotinoid Pesticides Residues by High Performence Liquid Chromatography with Molecularly Imprinting Solid Phase Extraction

以噻虫啉为模板分子制备了对吡虫啉、氯噻啉、噻虫啉具有特异性识别的分子印迹聚合物。功能单体与模板分子最佳摩尔比为：n（噻虫啉）∶n（甲基丙烯酸）∶n（苯乙烯）=1∶4∶4。动态吸附和选择性吸附表明,此印迹聚合物能够对目标物快速吸附,且有很好的选择性。Scatchard分析表明,此印迹聚合物对吡虫啉、氯噻啉、噻虫啉最大吸附量分别为31.7,36.7和45.3 mg/g。用此印迹聚合物作为固相萃取吸附剂处理加标样品,目标物的回收率为80.2%-98.8%,RSD为1.2%-4.5%（n=3）。本方法用于实际样品检测,获得了满意的结果。

A molecularly imprinted polymer（ MIP） for the selective solid phase extraction of imidacloprid, imidaclothiz,thiacloprid was synthesized by polymerization for 24 h using thiacloprid as template. Dynamic adsorption and selective adsorption test showed that the MIP could quickly adsorb the imidacloprid, imidaclothiz,thiacloprid,with good selectivity for targets. The maximum static adsorption capacity of MIP was 31. 7,36. 7 and 45. 3 mg / g,respectively. A molecularly imprinted solid phase extraction（ MIP-SPE） was developed to separate,clean up and enrich the thiacloprid,imidacloprid and imidaclothiz residue in paddy water,soil,rice,tomato,cucumber. The average recoveries were 80. 2%- 98. 8%,with relative standard deviation of 1. 4%- 4. 5%. The MIP-SPE was used to analyses the real samples,the result was satisfied.