在线测量发酵液葡萄糖浓度的过滤取样探头的研制

A Rapid Filtration Sampling Probe for Fermentation Broth On-line Measurement

设计了一种罐内快速过滤取样探头,以解决在线测量发酵参数过程中的发酵液连续取样问题。此探头由支撑体、旋塞阀、O型圈、过滤膜管、管盖组成,可以直接插入发酵罐内,能够承受发酵前期的灭菌高温,并能避免取样时杂菌入侵发酵液。过滤膜采用筒状陶瓷膜,罐内发酵液经错流过滤,可由蠕动泵抽取适量无菌的渗透液给分析仪。利用此装置对葡萄糖溶液进行了取样测试,结果表明,此装置适用于发酵液在线取样,取样速率为3.0 mL/min,葡萄糖透过率保持100%,探头引起的时间延迟约为2 min。

An inner-bioreactor rapid filtration sampling probe was designed to resolve the problems of continuous sampling the fermentation broth in on-line measurement the fermentation process. The probe consists of a support,a plug valve,an O-ring,a filter membrane and a pipe cover. It can be inserted directly into the bio-reactor withstanding high-temperature sterilization before fermentation,or preventing the bacteria＇s invasion during sampling. Fermentation broth in tank is cross-flow filtrated by a cylindrical ceramic membrane which is used as filtration membrane,and then the sterile permeated liquid is transported to the analyzer by a peristaltic pump. The sampling experiments using this device was described for sampling glucose solution indicating it is suitable for fermentation online sampling. The sampling rate is up to 3. 0 mL / min,glucose permeability maintains 100%,and the delay time caused by the probe is about 2 min.