2,5-己二酮对SK-N-SH细胞中神经生长因子表达水平的研究

Study on the effects of 2,5- hexanedione on the expression of nerve growth factor in SK-N-SH cells

目的分析2,5-己二酮对人SK-N-SH细胞中神经生长因子表达水平变化的研究,探讨正己烷中毒的分子机制。方法分别以0、10、20、40 mM的2,5-己二酮持续染毒12 h后,以Q-PCR及Western Blot方法检测各组人SKN-SH细胞中神经生长因子的变化趋势。结果 2,5-己二酮染毒后,人SK-N-SH细胞神经生长因子mRNA及其蛋白表达水平均明显下调,可见染毒后SK-N-SH后细胞分泌的神经生长因子是降低的。结论 2,5-己二酮可以引起人SK-N-SH细胞NGF表达水平降低,说明NGF表达降低在2,5-己二酮对人SK-N-SH细胞的早期毒性及慢性毒作用机制中起着重要作用。

Objective To analyze the effects of 2,5 - hexanedione （2,5 - liD） on the expression levels of nerve growth factor （NGF） in htmaan SK - N - SH cells, and to explore the molecular mechanism of n- hexane poisoning. Methods Human SK - N - SH cells were respectively administrated with 0, 10, 20 and 40 mM 2,5 - HI） for I2 h. Real - time quantitative PCR de- tecting system and Western Blot were used to detect the changing trends of NGF in each group. Results After exposure to 2, 5 - HD, NGF mRNA and protein expression levels in human SK - N - SH cells were significantly down - regulated. Conclu- sions 2,5 - HD can decrease the expression levels of NGF in human SK - N - SH cells. It suggests that down - regulated NGF expression plays an important role in the mechanisms of early toxicity and chronic toxicity of 2,5 - lid to human SK - N - SH cells.