摘要
目的分析胰岛素抵抗（IR）肥胖大鼠血清增食欲素A（orexinA，OXA）与胰岛素样生长因子1（insulin—likegmwthfactor1，IGF-1）的相关性，阐明IR病理过程中增食欲素A参与脂肪代谢的可能作用机制。方法6周龄sD雄性大鼠，随机分为普通饮食组（NC组）和高脂肪饮食组（HF组）；建立IR肥胖大鼠模型，钳夹技术评定。大鼠皮下注射OXA（0～10μM）；用酶联免疫吸附法检测血清OXA和IGF-1。结果sD大鼠喂养6周后，高脂膳食诱导的肥胖大鼠（HF组）机体内的胰岛素、胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白（LDL—C）均明显高于Nc组（P〈0．05）；HF组大鼠体内的葡萄糖输注率60-120（GIR60-120）明显降低；大鼠皮下注射OXA（10^-8M、10^-2M、10^-6M），NC组大鼠血清IGF-1水平分别降低25．0％、43．5％、55．6％，IR肥胖大鼠血清IGF-1分别降低11．3％、17．7％、27．4％；IR肥胖大鼠血清OXA与IGF-1、HDL～C呈负相关，相关系数r分别为-0．342、-0．226，P〈0．05；与胰岛素、TC、TG、LDL-C呈正相关，相关系数r分别为0．303、0．388、0．255、0．253，P〈0．05。结论高脂饮食诱导的IR肥胖大鼠血清内OXA与IGF-1密切相关；OXA减弱IGF-1的含量，进而参与肥胖大鼠机体内IR的发生。
Objective To investigate the correlation of orexin A （OXA） with insulin - like growth factor 1 （ IGF - 1 ） in besity rat with insulin resistance induced by high - fat diet. Methods Male Sprague - Dawley rats model with insulin re- sistance induced by high -fat diet was verified by euglycemic insulin clamp technique to determine the insulin resistance. The rats were treated with OXA（O to 10 -6 M） by hypodermic injection. The serum levels of OXA and IGF - 1 in St） rat were detected by luminescent immunoassay enzymic method. Results After 6 weeks high - fat diet, serum insulin, total choles- terol （TC） , triglyeride （TG） and low density lipoprotein （LDL- C） increased significantly in obesity rats with insulin re- sistance （P 〈0.05 ） ,the GIR60-120 in obesity rats was decreased significantly ,compared with normal rats. After treated with OXA （ 10-8 M, 10 -7 M and 10-6 M） ,the levels of serum IGF - 1 decreased 25.0% ,43.5% and 55.6% in normal rats, respectively,decreased 11.3% ,17.7% and 27.4% in obesity rats, respectively. The correlation coefficient between OXA and serum IGF - 1 ,high density lipoprotein （ HDL - C） in insulin resistance rats were - 0. 342 and - 0. 226, P 〈 0.05. The correlation coefficient between OXA and serum insulin ,TC, TG, LDL- C in insulin resistance rats were 0. 303,0. 388, 0. 255,0.253 ,P 〈 0.05. Conclusions High - fat diet could induce insulin - resistance and obesity in SD rat. OXA corre- lated with IGF - 1 in insulin - resistance in SD rat. That OXA down - regulated serum IGF - 1 would involve in insulin - re- sistance in high -fat diet induced obesity rat.
出处
《创伤与急危重病医学》
2014年第3期179-182,共4页
Trauma and Critical Care Medicine
基金
国家自然科学基金(30872724
81071460
81271996)
辽宁省科技攻关层次计划项目(201202292)
