从外邪刺激下小鼠肺组织细胞因子变化探讨正邪交争实质

Exploration on Conflict between Vital-Qi and Pathogens Based on Changes of Cytokines in Lung of Mice Under Abnormal Environment

目的:通过观察不同异常环境下小鼠肺组织TNF-α、IL-1β、SOD及MDA的表达,探讨正邪交争的内在物质基础。方法:将实验组小鼠分别置于有风、高湿以及寒冷的环境下,每天连续刺激2 h,连续刺激4 d,空白组不作处理。第4 d刺激结束后立即处死动物,取肺组织,观察肺组织病理改变,用ELISA法检测肺组织中TNF-α、IL-1β、SOD、MDA的含量。结果:实验组各组小鼠肺组织TNF-α、IL-1β、MDA、SOD表达水平明显高于空白组,具有统计学差异(P<0.05)。结论:SOD可在异常环境下代偿性增高,以降低肺组织的损伤,是一种保护性反应,表明正邪交争理论存在一定的生物学物质基础。

Objective： To explore the conflict between the vital-qi and pathogens via observing the changes of the cytokines in the lung of mice under the abnormal environment. Methods： Kunming mice were randomly divided into 4 groups： the control group（ KB）,the H group,the W group,the T group. The control group did not receive any intervention. The H group was exposed to the environment of no wind,temperature（ 20 ± 2） ℃,relative humidity（ 85 ± 2） %. The T group was exposed to the environment of no wind,temperature（ 0 ± 2） ℃,relative humidity 40% ～ 50%. The W group was exposed to the environment of wind speed 2m /s,temperature（ 20 ± 2） ℃,relative humidity 40% ～ 50%. The stimulation time was 2 hours a day for 4 days. The lung tissue was collected and lung histopathologic changes and expressiong of TNF-α,IL-1β,SOD and MDA were observed. Results： Expressions of TNF-α,IL-1β,SOD and MDA in the experimental mice were raised compared with those of control group（ P 0.05）. Conclusion： SOD can increase when the body is composed to the abnormal environment to reduce the damage. It＇s a way to against the exogenous pathogenic factors. The confliction between the vital-qi and pathogens has certain biological material.