针刺对实验性脑出血大鼠炎性反应的时效研究

Study of Acupuncture Treatment Timing on Inflammation of Experimental Cerebral Hemorrhage Rats

目的:观察针刺对脑血肿早期周围组织病理形态结构、炎症反应的影响,探讨不同时机介入针刺对脑出血病理状态下的脑保护作用。方法:采用自体血注入尾状核模型大鼠,随机分为正常组、模型组和四个针刺组。针刺组分别于造模成功后3 h、9 h、24 h、48 h开始针刺,每日针刺2次,连续针刺3 d,针刺组与模型组均在造模成功后5 d处死,断头取脑,观察相关指标。结果:针刺能改善脑血肿周围组织病理改变,减轻脑水肿,改善神经功能缺损情况,降低炎症反应相关因子肿瘤坏死因子-α(TNF-α)、细胞间黏附分子-1(ICAM-1)的表达,这些作用均与介入时机正性相关。结论:针刺能减轻脑出血大鼠脑组织炎症反应,提示超早期介入能获得更好疗效。

Objective： To observe the effect on inflammation and pathological structure around hematoma and study protect effect against cerebra of acupuncture intervention timing for cerebral hemorrhage pathologic state and to discuss the best acupuncture intervention timing and major mechanism. Methods： An intracerebral hemorrhage rat model was made by injecting autologous blood into caudate nucleus,and the rats were randomly arranged into control group,model group and four acupuncture groups. Begin acupuncture in different time points（ 3 h,9 h,24 h and 48 h） after hematoma formation continued three days,twice a day.Model group and four acupuncture groups were all killed after model was successful and brains were removed to observe related indicators. Results： Acupuncture can improve organization pathological changes around hematoma,reduce brain edema,improve nerve function and reduce expression of inflammation-related factors TNF-α and ICAM-1. All these effects are associated with intervention timing. Conclusion： Acupuncture can alleviate inflammation response of organizations of rat with cerebral hemorrhage,and intervene sooner,the results better.