正交设计优选炮姜中新生成分姜酮的超声提取工艺

Optimization of ultrasonic extracting techniques of zingerone in processed ginger by orthogonal test

目的优选炮姜中新生成分姜酮的超声提取工艺。方法采用UPLC法测定炮姜中新生成分姜酮的含量,色谱柱为Waters Acquity BEH C18(100 mm×2.1 mm,1.7μm);流动相为乙腈—水,梯度洗脱;流速0.3 mL·min-1,检测波长280 nm,柱温30℃。采用正交设计法,考察乙醇浓度、倍数、提取时间、提取次数对姜酮的含量的影响。结果乙醇倍数与提取时间对提取结果有显著性影响(P<0.05),综合分析姜酮的最佳超声提取工艺为:80%乙醇,10倍量,提取1次,提取时间40 min。结论优选的提取工艺合理可行,可为炮姜的质量控制和进一步开发提供依据。

Objective Study on the ultrasonic extracting techniques of zingerone in processed ginger.Methods UPLC analysis was performed to analyze the content of zingerone in processed ginger,the chromatographic column was Waters Acquity BEH C18（100 mm ×2.1 mm,1.7 μm）,with acetonitrile-water as the mobile phase at 0.3 mL·min-1,and detection wavelength was 280 nm,column temperature at 30℃; the orthogonal test was employed to study the effect of quantities of ethanol concentration,volume of ethanol,extraction time and times.Results The volume of ethanol and extraction time had significant influence on the extraction effect（P 0.05）,the optimal ultrasonic extracting condition for zingerone were as follows：80% ethanol,extracted one time for 40min with 10-fold ethanol.Conclusion Optimized extraction condition was reasonable and feasible,and can provide a basis for the quality control and further development of processed ginger.