高效液相法测定复方氟苯尼考注射液中氟苯尼考的含量

Determination of florfenicol content in florfenicol injection by HPLC

建立了测定氟苯尼考注射液中氟苯尼考含量的高效液相色谱法。采用C18色谱柱(4.6 mm×150 mm,5μm),流动相为甲醇∶水(pH值为3.0)为40∶60(v/v),流速为1 mL/min,检测波长为222 nm,进样10μL,柱温为室温。氟苯尼考浓度在0.1-3 mg/mL范围内,峰面积与浓度的线性关系良好(R2=0.999 6);平均回收率为99.49%,RSD为1.12%(n=4)。此法简便、快速、灵敏度高、重现性好。

A method for the content determination of florfenicolm injection by HPLC was established. Waters C18（4.6 mm×150 mm,5 μm）column was used and the samples were detected by UV detector at 222 nm with water（pH 3.0, adjusted by0.1 mol/L Phosphate）∶methanol（40∶60）as the mobile phase. The flow rate was 1.0 mL/min with the injection volume of 10μL at room temperature. There was no interference occurred from impurities. The calibration curve was linear in the range of 0.1-3 mg/mL（R^2=0.999 6）, and the average recovery was 99.49% with RSD of 1.12%. This established method is simple, rapid, sensitive and accurate.