生化黄腐酸对3种肿瘤细胞体外增殖影响的初步研究

The Preliminary Study of Biochemical Fulvic Acidon the Proliferation of Three Types of Tumor Cells in Vitro

本试验采用四甲基偶氮唑蓝(MTT)法,研究了生化黄腐酸对3种肿瘤细胞(人胃癌细胞株SGC、人肝癌细胞株SPCA和人肺癌细胞株BEL)在体外增殖的影响。在3种不同肿瘤细胞培养液内分别加入6种不同浓度的生化黄腐酸,用酶标仪测定3种不同培养肿瘤细胞的吸光值(OD值),计算生化黄腐酸对3种不同肿瘤细胞的抑制率。试验结果表明:在各个浓度下,生化黄腐酸对3种不同肿瘤细胞的抑制率均低于50%,属于非敏感型药物。生化黄腐酸对肿瘤细胞的体外增殖没有明显的直接抑制作用,且在某种浓度下,生化黄腐酸可能对肿瘤细胞有促进生长的作用。

In this experiment, three kinds of cancer cells, SGC, SPCA and BEL as the object was studied The [3-（4,5-Di-methyl-2-Thiazolyl）-2,5-Diphenyl Tetrazolium Bromide] method （MTT） was applied to study on the inlfuence of three kinds of tumor cell proliferation in vitro. Six different concentrations of biochemical fulvic acid were added into the three different cultured tumor cells. The OD values of three different cultured tumor cells were measured, and the con-centration of cell inhibition rate of each was calculated. The results showed that the inhibitions of biochemical fulvic acid on three different tumor cells were less than 50%in all concentrations, indicating that they were not sensitive. The proliferation of three different tumor cells did not induce directly inhibited by biochemical fulvic acid. In addition, bio-chemical fulvic acid could promot growth of the tumor cells to some extent.