基于石墨烯分子印迹电化学传感器测定氯霉素

Electrochemical Sensor for Chloramphenicol Based on a Novel Graphene Oxide-Molecular Imprinted Polymer Composite

采用改进的Hummers法合成氧化石墨烯,通过共沉淀作用在氧化石墨烯表面生成磁性Fe3O4纳米微粒,经硅烷化修饰巯基,将金纳米颗粒自组装到复合材料中,得到磁性氧化石墨烯复合金纳米颗粒,将其滴涂在金电极表面,以氯霉素为模板分子,通过溶胶-凝胶法将分子印迹膜修饰到金电极上,制得氯霉素分子印迹电化学传感器,并对制备电化学传感器进行条件优化和电化学性能研究。结果显示,基于石墨烯分子印迹电化学传感器测定氯霉素的线性范围为2.5×10-9-5.0×10-6mol/L,检出限为8.0×10-10mol/L。

The modified Hummers method was used to synthetize graphene oxide, a novel nanocomposite of magnetic graphene ox idegold nanopartic/es was synthesized using coprecipitation and selfassembly technique, and the product was dropped on the surface of gold electrode. Then a molecular imprinted polymer film was fabricated by solgel technique in the presence of chloramphenieol onto a gold electrode surface. By optimizing experimental conditions, a linear concentration range of 2.5 x 10^- 9 - 5.0 x 10^- 6 mol/L was ob tained with a detection limit of 8.0 x 10^-10 mol/L. The new electrochemical sensor also exhibited high selectivity and excellent sensitivi ty, and it was successfully applied to the detection of ehloramphenicol rapidly in spiked samples.