NaCl对拟南芥VHA-c基因的调节

The Regulation of Arabidopsis Thaliana VHA-c by NaCl

将烟草叶片以不同浓度的NaCl溶液处理下,研究转三磷酸腺苷酶(V-ATPase c)亚基基因(VHA-c)的β-葡萄糖苷酸酶(GUS)基因表达水平的变化。结果表明,V-ATPase c2亚基基因(VHA-c2)和V-ATPase c3亚基基因(VHA-c3)的表达活性有所降低,V-ATPase c5亚基基因(VHA-c5)的表达被50 mmol/L的NaCl促进,被200 mmol/L的NaCl抑制,说明VHA-c2、VHA-c3和VHA-c5均可被NaCl溶液调控,且VHA-c2的表达不存在与NaCl溶液浓度的协同关系,VHA-c3和VHA-c5对较低浓度的NaCl溶液胁迫更为敏感,对高浓度的NaCl处理并不敏感。

After tobacco leaves are treated with NaC1 solutions of different concentration,the GUS transcription level in VHA-c-GUS transgenic tobacco leaves is studied. It is shown that the transcription level of VHA-c2 and VHA-c3 decreases slightly,while VHA-c5 increases in 50 mmol/L of NaC1 solution but inhibited by 200 mmol/L of NaC1 solution. This shows that VHA-c2, VHA-c3 and VHA-c5 can be regulated by NaC1 solution. The expression of VHA-c2 has no coordination with the concentration of NaC1 solution, however VHA-c3 and VHA-c5 are sensitive to NaC1 solution of lower concentration but is insensitive to NaC1 solution of higher concentration.