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NaCl对拟南芥VHA-c基因的调节

The Regulation of Arabidopsis Thaliana VHA-c by NaCl
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摘要 将烟草叶片以不同浓度的NaCl溶液处理下,研究转三磷酸腺苷酶(V-ATPase c)亚基基因(VHA-c)的β-葡萄糖苷酸酶(GUS)基因表达水平的变化。结果表明,V-ATPase c2亚基基因(VHA-c2)和V-ATPase c3亚基基因(VHA-c3)的表达活性有所降低,V-ATPase c5亚基基因(VHA-c5)的表达被50 mmol/L的NaCl促进,被200 mmol/L的NaCl抑制,说明VHA-c2、VHA-c3和VHA-c5均可被NaCl溶液调控,且VHA-c2的表达不存在与NaCl溶液浓度的协同关系,VHA-c3和VHA-c5对较低浓度的NaCl溶液胁迫更为敏感,对高浓度的NaCl处理并不敏感。 After tobacco leaves are treated with NaC1 solutions of different concentration,the GUS transcription level in VHA-c-GUS transgenic tobacco leaves is studied. It is shown that the transcription level of VHA-c2 and VHA-c3 decreases slightly,while VHA-c5 increases in 50 mmol/L of NaC1 solution but inhibited by 200 mmol/L of NaC1 solution. This shows that VHA-c2, VHA-c3 and VHA-c5 can be regulated by NaC1 solution. The expression of VHA-c2 has no coordination with the concentration of NaC1 solution, however VHA-c3 and VHA-c5 are sensitive to NaC1 solution of lower concentration but is insensitive to NaC1 solution of higher concentration.
出处 《济南大学学报(自然科学版)》 CAS 北大核心 2014年第5期342-346,共5页 Journal of University of Jinan(Science and Technology)
基金 国家自然科学基金(31060105) 内蒙古自然科学基金(2010Zd13)
关键词 拟南芥 VHA—c GUS NACL 盐胁迫 Arabidopsis thalian VHA-c GUS NaCl salt treatment
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  • 1刘友良,汪良驹.植物对盐胁迫的反应和耐盐性[M],2版.植物生理与分子生物学.北京:科学出版社,1998:752-769.
  • 2王东明,贾媛,崔继哲.盐胁迫对植物的影响及植物盐适应性研究进展[J].中国农学通报,2009,25(4):124-128. 被引量:184
  • 3DUPONT F M, IN : COOKE D T, CLARKSON D T,et al. Transport and receptor proteins of plant membranes[ M ]. New Ynrk:Plunum Press, 1992:91.
  • 4NAKAMURA Y, KASAMO K,SHIMOSATO N,et al. Stimulation of the extrusion of protons and H -ATPase activities with the decline in pyrophosphatase activity of the tonoplast in intact mung bean roots under high-NaC1 stress and its relation to external levels of Ca2 + ions [ J ]. Plant Cell Physiol, 1992,33 : 139 - 149.
  • 5MATSUMOTO H, CHUNG G C. Increase in proton-transport activi- ty of tonoplast vesicles as an adaptive response of barley roots to NaC1 stress [ J ]. Plant Cell Physiol, 1988,29 : 1133 - 1140.
  • 6LOW R, ROCKED B, KRISH M, et al. Early salt stress effects on the differential expression of vacuolar H -ATPase genes in roots and leaves of mesembryanthemum erystallinum [ J ]. Plant Physiol, 1996,110( 1 ) :259 -265.
  • 7ZHANG W, DIAO F, YU B, et al. H -ATPase and H -transport activities in tonoplast vesicles from barley roots under salt stress and influence of calcium and abscisic acid [ J . J Plant Nutri, 1998,21 ( 3 ) :447 - 458.
  • 8ZHANG W,ZHANG F,SHEN Z,et al. Changes of H pumps of tonoplast vesicles from wheat roots in vivo and in vitro under alumi- num treatment and effect of calcium[ J]. J Plant Nutri,1998b,21 : 2515 -2516.
  • 9SCHNITZER D, T SEIDEL H. The cellular energization state af- fects peripheral stalk stability of plant vacuolar H + -ATPase and impairs vacuolar acidification [ J ]. Plant Cell Physiol, 2011, 52 (5) :946 - 956.
  • 10BRUX A,LIU T Y. Reduced V-ATPase activity in the trans-golgi network causes oxylipin-dependent hypocotyl growth inhibition in arabidopsis [ J ]. The Plant Ce11,2008,20 (4) : 1088 - 1100.

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