HPLC法同步测定三七粉中人参皂苷Rg_1、Rb_1及三七皂苷R_1的含量

Synchronous Determination of Ginsenoside Rg_1, Ginsenoside Rb_1 and Notoginsenoside R_1 in Radix Notoginseng Powder by HPLC

目的：采用HPLC法同步测定三七粉中人参皂苷Rg1、Rb1及三七皂苷R1含量。方法：采用SGE protecol C18（5μm，4.6×250mm）色谱柱，流动相为乙腈-水梯度洗脱（0-12min，19∶81；12-60min，19-36∶81-64），检测波长为203nm，流速1.0mL/min，进样量20μL。结果：人参皂苷Rg1、人参皂苷Rb1、三七皂苷R1分别在25.625～430mg/L、26.875～410mg/L、10.625～170mg/L范围呈线性，相关系数r分别为0.9999、0.9998、0.9996；该方法重复性及回收率均符合要求。结论：本法用于同步测定三七粉中人参皂苷Rg1、Rb1及三七皂苷R1的含量，具有简便、准确、高效等特点。

Objective： This study has been to establish a method for synchronous determination of ginsenoside Rg1, ginsenoside Rb1 and notoginsenoside R1 in radix notoginseng powder. Method： SGE protecol C18（5μm,4.6×250mm）chromatographic column was used, with mixtures of acetonitrile and water as mobile phase in gradient mode （0-12min,19∶81; 12-60min, 19-36∶81-64）, the wavelength was 203nm, the flow rate was 1.0mL/min, the injection volume was 20μL. Results： The linear range of the three saponins is 25.625 - 430mg/L, 26.875 - 410mg/L, 10.625 - 170mg/L respectively, and the correlation coefficient r is 0.9999, 0.9998, 0.9996 respectively. Repeatability and recovery rate are good. Conclusion： The presented method is a simple, accurate, efficient method for synchronous determination ginsenoside Rg1, ginsenoside Rb1 and notoginsenoside R1 in radix notoginseng powder.