高速逆流色谱(盐溶液体系)分离制备白芍中的芍药苷

Separation and preparation of paeoniflorin from Paeonia lactiflora Pall.by HSCCC with salt solution system

建立应用高速逆流色谱分离纯化芍药中的芍药苷的方法。以正丁醇-乙酸乙酯-5%Na2SO4(4:1:5,V/V)为两相溶剂体系,在流速为2.0 mL/min、主机转速为800 r/min、检测波长为254 nm的条件下进行分离,从100 mg芍药粗提物中一次性分离制备得到56.13 mg芍药苷,高效液相色谱分析其纯度在98%以上,通过质谱、核磁共振氢谱和核磁共振碳谱鉴定化合物的结构。该方法简便、快捷且重现性好,适合芍药苷的制备型分离。

We established a method of separation and preparation of paeoniflorin from Paeonia lactiflora Pall.by highspeed counter current chromatography （HSCCC） with salt solution system.The separation was performed with butanolethyl acetate-5％ Na2SO4 （aq） （4∶1∶5,V/V） as a two-phase solvent system,flow rate of 2.0 mL/min,rotation number of 800 r/min and detection wavelength of 254 nm.We obtained paeoniflorin of 56.13 mg from the crude extracts of 100 mg at one time.Its purity was more than 98％,which was determined by high performance liquid chromatography（HPLC）.The structure of the target compound was identified by electrospray ionisation mass spectrometry （ESI-MS）,1H-nuclear magnetic resonance （1H-NMR） and 13C-nuclear magnetic resonance （13C-NMR）.The method is simple and reproducible and suitable for separation and preparation of paeoniflorin.