脂肪酶蛋白质工程研究进展

Research progress on protein engineering of lipases

脂肪酶在油脂化学、食品工业、有机合成和生物柴油等多个领域有着广泛应用。利用蛋白质工程在分子水平上修饰脂肪酶,可以提高酶活、稳定性和其它催化性质。该文综述了蛋白质工程的基本概念和利用不同方法开发新突变脂肪酶的应用。

Lipases have been widely used in oil chemicals,food industry,organic synthesis and biodiesel industry,etc. Protein engineering was used to modify the lipase on a molecular level,for examples,improving the activity,stability and other catalytic properties of lipases. This article reviewed the concepts of protein engineering and the discovery examples of novel lipases and their variants by protein engineering.