摘要
HLA Ⅱ类抗原在外来抗原提呈中起重要作用。在DNA水平进行Ⅱ类抗原基因分型有更强的多态性鉴别能力,有利于检出新的特异性。根据白种人纯合分型细胞(HTC)和中国人部分纯合细胞DRB和DQA基因的RFLP格局,我们对上海地区90名随机健康人进行了HLA-D区基因分型,并将结果与血清学分型相比较,证明了它的可靠性和优越性。此外,通过与白种人格局相比,我们还发现了中国人特有的HLA-D区RFLP格局。
The HLA class Ⅱ antigens play very an important role in presenting foreign antigens. The class Ⅱ gene typing at the DNA level possesses higher capability to recognize polymorphism so that it can disclose new specificities. According to the RFLP patterns of DRB and DQA genes for Caucasian HTC and some Chinese homozygous cells, we typed 90 randomly sampled healthy Chinese Hans in Shanghai area, and compared the results obtanied with those from serological typing. In addition to being reliable and superior to serological typing, we found some unique RFLP patterns of the HLA-D region in Chinese Han population based on comparison with Caucasian patterns.
关键词
基因分型
印迹法
RFLP
汉族
Gene typing, Southern blot, Restriction fragment lenth polymorphism (RF-LP)
