基于形态特征和ITS序列分析秦巴山区黑木耳主要栽培种的亲缘关系

Analysis of the Genetic Relationships of Main Auricularia auricular Cultispecies in Qinba Mountainous Area Based on ITS Sequence

采用CTAB法提取黑木耳菌株的DNA,进行PCR扩增,扩增产物进行测序。耳2和标准菌株Auricularia auricula-judae HF-8(GQ168492)聚类在同一分支上;耳913和标准菌株Auricularia auricula-judae YBS-7(GQ168503)聚类在同一分支上,且神农A8与新科、耳2、耳913亲缘关系相对较远。耳97-2和标准菌株Auricularia auricula-judae NW482(EU520194)聚类在同一分支上;耳268和标准菌株Auricularia auricula-judae NW491(EU520160)聚类在同一分支上,且秦单4#与耳97-2、耳268亲缘关系相对较远。新科、耳2、耳913、神农A8此4个菌种亲缘关系比较近;耳268、秦单4#、耳97-2等3个菌种亲缘关系较近。通过黑木耳菌株的形态观察及ITS序列分析,得到两株亲缘关系较远的优势菌株秦单4#和神农A8。

Analyzing the genetic relationships of the main Audcularia audcular cultispecies in Qinba mountainous area by ITS sequence so as to lay a theoretical foundation to genetics and breeding.Using CTAB method to extract genomic DNA from the bacterial strain,Auricularia auricular,and amplifying and sequencing PCR products ;A uricularia auricula-judae 2 and Auricularia auricula-judae HF-8 （GQ168492） were clustered in the same branch,Auricularia auric ula-judae 913 and A uric ularia auric ula-judae YB S-7 （GQ 168503） were clustered in the same branch,but the genetic relationship between Auricularia auricula-judae Shennong A8 and the A uricularia auricula-judae 2,Auricularia auricula-judae 913 were relatively alienative.A uricularia auricula-judae 97-2,Auricularia auricula-judae NW482 （EU520194）,the Auricularia auriculajudae 268 and Auricularia auricula-judae NW491 （EU520160） were clustered in the same branch.However the genetic relationships among Auricularia auricula-judae Qindan 4,Auricularia auricula-judae 97-2,Auricularia auricula-judae 268 were relatively alienative.The genetic relationships among A uricularia auricula-judae Xinke,A uricularia auricula-judae 2,A uricularia auricula-judae 913 and Auricularia auricula-judae Shennong A8 were close.However,the relationships between A uricularia auric ula-judae 268,A uricularia auric ula-judae Qindan 4# and Auricularia auricula-judae 97-2 were quite far.Through the morphologic and ITS analysis of Auricularia auricular strains,two dominant strains,Auricularia auricula-judae Qindan 4# and A uricularia auricula-judae Shennong A8,have alienative relationship.Our findings established a solid foundation to genetics and breeding.