多指标正交试验优选莲松异搏停片中延胡索、苦参醇提工艺

Optimization of Extraction Technology for Corydalis Rhizoma and Sophorae Flavescentis Radix in Liansong Yiboting Tablets by Orthogonal Design with Multi-indexes

目的：优选莲松异搏停片中延胡索、苦参的提取工艺.方法：以延胡索乙素、苦参碱和氧化苦参碱提取量为指标,通过正交试验和单因素试验考察乙醇体积分数、溶剂用量、提取次数和时间对醇提工艺的影响.采用HPLC测定指标成分含量,流动相乙腈（A）-磷酸二氢铵溶液（B）梯度洗脱（0～10 min,10％A;10～25 min,10％～37％A;25 ～ 45 min,37％A）,检测波长220 nm.结果：最佳提取工艺为加6倍量70％乙醇提取3次,每次2h;延胡索乙素提取量332.04 mg,苦参碱和氧化苦参碱总量3 192.08 mg.结论：优选的提取工艺稳定可行,建立的色谱条件可较好地分离3个指标成分.

Objective - Radix in Liansong Yiboting To optimize extraction technology of Corydalis Rhizoma and Sophorae Flaveseentis tablets. Method： With extracting amounts of matrine, oxymatrine and tetrahydropalmatine as indexes, orthogonal test and single factor test was adopted to optimize extraction process by taking ethanol concentration, solid-liquid ratio, extracting times and time as factor. HPLC was employed to determine contents of index components with mobile phase of acetonitrile （A） -ammonium dihydrogen phosphate solution （B） for gradient elution （0-10 min, 10% A; 10-25 min, 10%-37% A; 25-45 rain, 37% A） and detection wavelength at 220 nm. Result： Optimum extraction technology was as following： extracted 3 times with 6 times the amount of 70% ethanol for 2 h each time. Extracting amount of tetrahydropalmatine was 332.04 mg, total extracting amount of matrine and oxymatrine was 3 192.08 mg. Conclusion： This optimized extraction technology was stable and feasible, these established chromatographic conditions had a good separation for three components.