芪苈强心胶囊的化学成分(三)

Chemical Components from Qili Qiangxin Capsule( Ⅲ)

目的：研究复方中药芪苈强心胶囊的化学成分,从而明确芪苈强心胶囊的药效物质基础.方法：使用硅胶柱色谱、凝胶柱色谱、中低压液相色谱和制备高效液相色谱等手段,对芪苈强心胶囊大孔树脂95％,50％,30％醇洗部分的化学成分进行分离和鉴定.结果：从这三段洗脱物中分离纯化得到10个单体化合物.利用1D,2D核磁共振光谱全部鉴定了其结构.分别为人参皂苷Rd（1）,20（S）-人参皂苷Rh1（2）,20（S）-人参皂苷Rg2（3）,毛蕊异黄酮（4）,杠柳苷△5-pregnene-3β,20（S）-diol-20-O-β-D-glucopyranosyl-（1 →6）-β-D-glucopyranosyl-（1→2）-β-D-digitalopyranoside（5）,黄芪皂苷Ⅰ（6）,山柰酚-3-O-β-D-芸香苷（7）,橙皮苷（8）,迷迭香酸甲酯（9）,迷迭香酸乙酯（10）.结论：化合物1～6,8～ 10均为首次从芪苈强心胶囊中分离得到.

Objective： To study the chemical components of Qili Qiangxin capsule and to prove its material basis for efficacy. Method： The compounds were isolated from fractions eluted by 95% ethanol, 50% ethanol and 30% ethanol on silica gel, sephadex LH-20, MPLC and PHPLC repeatedly. Result： Ten compounds were isolated and their structures were elucidated on the basis of chemical methods and spectral analyses. They were identified as ginsenoside Rd （ 1 ） , 20 （S） -ginsenoside RhI （2） , 20 （S） -ginsenoside Rg2 （3）, calycosin （4）,△5-pregnene-3β, （S） -diol-20-O-β-D-glucopyranosyl- （ 1→6 ） -β-D-glucopyranosyl- （1→2） -β- D-digitalopyranoside （ 5 ）, astragaloside I （ 6 ）, kaempferol-3-O-β-D-rutinoside （ 7 ）, narirutin （ 8 ）, methyl rosmarinate （9） and ethyl rosmarinate （10）. Conclusion： The compounds 1-6, 8-10 are identified from Qili Qiangxin capsule for the first time.