二神丸中药物炮制前后化学成分含量变化

Contents Change of Chemical Compositions in Ershen Pills Before and After Preparation

目的：建立同时测定二神丸中3种成分含量的HPLC,比较二神丸中药物炮制前后补骨脂素、异补骨脂素和去氢二异丁香酚含量的变化.拟阐明炮制对方中药物化学成分的影响.方法：二神丸中的药物补骨脂盐炙,肉豆蔻麸煨,采用HPLC测定2味药炮制前后3种成分的含量变化.结果：该测定方法补骨脂素的线性范围0.164 ～2.05 μg,回收率101.76％,RSD 2.02％;异补骨脂素的线性范围0.115～1.44 μg,回收率96.15％,RSD 1.55％;去氢二异丁香酚的线性范围0.086 4～1.08 μg,回收率99.61％,RSD 1.96％.二神丸中药物炮制后,补骨脂素和异补骨脂素的含量均降低,去氢二异丁香酚的含量亦降低.结论：该方法简单准确,重复性好.二神丸中药物炮制后补骨脂素、异补骨脂素和去氢二异丁香酚的含量均发生了一定变化.

Objective： To establish an HPLC method for simultaneous determination of three components in Ershen pills and compare the content changes of psoralen, isopsoralen and dehydrodiisoeugenol in this prescription before and after processing, and to investigate the influence of processing on chemical compositions. Method： Psoralea was stir-fried with salt and Nutmeg was roasted with wheat bran. HPLC was applied to determine the content changes of psoralen, isopsoralen and dehydrodiisoeugenol in this prescription before and after processing. Result： The average recoveries of psoralen, isopsoralen and dehydrodiisoeugenol were 101.76%, 96. 15% , 99.61% , with RSD being 2.02% , 1.55% , 1.96%. The psoralen, isopsoralen and dehydrodiisoeugenol samples showed a good linear relationship in the ranges of 0. 164-2.05, 0. 115-1.44, 0. 086 4-1.08 μg, respectively, in the chosen HPLC conditions. After preparation, the three compositions were reduced. Conclusion： The method is simple, accurate and reproducible. The contents of psoralen, isopsoralen and dehydrodiisoeugenol in Ershen pills can be changed after processing.