不同采收时期牻牛儿苗中4种酚酸类活性成分的动态分析

Dynamic Variation of 4 Active Component of Phenolic Acids in Erodium stephanianum from Different Collection Time

目的：测定不同采收时期牻牛儿苗中活性成分没食子酸、原儿茶酸、柯里拉京及鞣花酸的含量,为最佳采收期的确定提供依据.方法：采用RP-HPLC,AgilentTC-C18色谱柱（4.6 mm×250 mm,5μm）,0.4％磷酸水溶液-甲醇为流动相梯度洗脱,流速0.8 mL·min^-1,检测波长259 nm,柱温30℃.结果：没食子酸,原儿茶酸,柯里拉京,鞣花酸的线性范围分别为0.492 ～4.920 g·L^-1（r =0.999 3）,0.497 ～4.970 g·L^-1（r =0.999 7）,0.995 ～9.950 g·L^-1（r =0.999 8）,0.989 ～9.890 g·L^-1（r=0.9997）;平均回收率分别为100.46％（RSD 2.1％）,99.78％ （RSD 1.3％）,100.18％ （RSD 1.0％）,100.21％（RSD 1.4％）.结论：牻牛儿苗中没食子酸及原儿茶酸的含量在五月末达到最大值,柯里拉京及鞣花酸的含量在六月末达到最大值.4种酚酸活性成分总量在六月末达到最高.

Objective： To determine the contents of the active components gallic acid, protocatechuic acid, corilagin and ellagic acid in Erodium stephanianum from different collection time and to define the best collection time for this herb. Method： The RP-HPLC method was applied and the separation was performed on an Agilent TC-C18 analytical column （4.6 mm × 250 mm, 5 μm）. The mobile phase was methanol （A） -water containing 0.4% H3PO4 （B） with gradient elution mode at the flow rate of 0.8 mL min^-1 The detection was set at 259 nm, and the column temperature was 30℃. Result： The liner ranges of gallic acid, protocatechuic acid, corilagin and ellagic acid were 0. 492-4. 920 g ·L^-1 （r =0. 999 3）, 0. 497-4. 970 g ·L^-1 （r =0. 999 7）, 0. 995- 9. 950 g ·L^-1 （ r = 0. 999 8 ）, 0. 989-9. 890 g·L^-1 （ r = 0. 999 7 ）, respectively. The average recoveries were 100.46% （RSD 2.1%）, 99.78% （RSD 1.3%）, 100.18% （RSD 1.0%）, 100.21% （RSD 1.4%）, respectively. Conclusion： The content of gallic acid and protocatechuic acid, in E. stephanianum increased to the maximum value in late May, the content of corilagin and ellagic acid increased to the maximum value in late June. Four acids active compounds increased to the maximum in late June.