灯盏花素对2型糖尿病大鼠糖脂代谢的影响

Effect of Breviscapine on Glucose and Lipid Metabolism in Type 2 Diabetes Mellitus Rats

目的:观察灯盏花素对2型糖尿病(T2DM)大鼠糖脂代谢的影响。方法:高脂高糖饮食联合链尿佐菌素(STZ)建立T2DM大鼠模型,将成模大鼠随机分为模型组、灯盏花素低、高剂量组(100,200 mg·kg-1·d-1),并设正常对照组。腹腔注射(ip)灯盏花素14 d后,比较各组大鼠的一般状态、空腹血糖(FBG)、空腹胰岛素(INS)、血脂[总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)]及脂联素(APN)水平,并制作大鼠胰腺组织病理切片。结果:与正常组比较,模型组大鼠糖尿病状态明显,血清FBG,INS,TC,TG及LDL-C水平升高,APN水平降低(P<0.01),胰腺组织病理损害明显;与模型组比较,低、高剂量组大鼠一般状态有所改善,血清FBG,INS,TC,TG及LDL-C水平降低,APN水平升高(P<0.01或P<0.05),胰腺组织病理损害减轻。结论:灯盏花素可能通过改善T2DM大鼠APN低水平状态和胰岛素抵抗来改善糖、脂代谢紊乱。

Objective： To study the effect of breviscapine on glucose and lipid metabolism in type 2 diabetes mellitus （T2DM） rats. Method： The T2DM model rats were induced by feeding of high-fat and high- carbohydrate diet and injecting streptozotocin and then were randomly divided into the model group （MG） , the low and high dose group （100, 200 mg·kg^-1·d^-1, LG, HG）, A normal control group was also set up （NG）. The drugs were given for 14 days by intraperitoneal injection. Then the general status, the fasting blood glucose （FBG）, insulin （ INS）, blood lipid [ total cholesterol （ TC）, triglycerides （TG）, and low-density lipoprotein cholesterol （LDL-C）] and adiponectin （APN） levels were compared between each group. The rat pancreatic tissue pathological section were also made. Result： Compared with NG, there were obvious symptoms of diabetes in MG, levels of FBG, INS, TC, TG, LDL-C significantly increased and levels of APN decreased in MG （P 〈 0.01 ）. Compared with MG, the general status improved in both LG and HG, levels of FBG, INS, TC, TG, LDL- C significantly decreased and levels of APN increased in both LG and HG （P 〈0. 01 or P 〈0.05）. Conclusion： Breviscapine may adjust the disturbance in the glucose and lipid metabolism by improving the low adiponectin levels and insulin resistance in T2DM rats.