鼻渊舒对慢性鼻-鼻窦炎模型鼻窦IL-1β mRNA表达及NF-κB DNA结合活性的影响

Influence of Biyuanshu Oral Solution on Expression of IL-1β mRNA and NF-κB DNA Binding Activity of Nasal Sinuses Mucosa Epithelial in Rabbit Chronic Rhinosinusitis Model

目的通过观察鼻渊舒对兔CRS模型鼻窦黏膜上皮NF-κB活性、IL-1βmRNA表达的影响,探索其治疗CRS的机制。方法健康新西兰兔100只,按每组20只,随机分为正常、假手术、模型、鼻渊舒、西药组后,建立CRS模型;鼻渊舒组予鼻渊舒口服、西药组给予克拉霉素灌胃,共14天。HE染色观察鼻窦黏膜病理改变,凝胶电泳迁移分析(EMSA)检测鼻窦黏膜上皮NF-κB的DNA结合活性,实时定量PCR检测IL-1βmRNA表达。结果模型组鼻窦黏膜呈慢性炎症表现;NF-κB DNA结合活性及IL-1βmRNA表达较正常组显著增高(P<0.01)。鼻渊舒组鼻窦黏膜上皮得到较好修复,炎细胞浸润不明显,腺体及杯状细胞少见增生;NF-κB DNA结合活性及IL-1βmRNA表达显著低于模型组(P<0.05),与正常组比较无显著差异(P>0.05)。结论鼻渊舒治疗CRS的机制可能与降低鼻窦黏膜上皮NF-κB DNA结合活性,进而抑制IL-1β基因转录有关。

Objective To investigate the influence of Biyuanshu Oral Solution（BYS） on nasal sinuses mucosa epithelial NF - KB activity and IL-1β mRNA expression in rabbit chronic rhinosinusitis model, and explore its possible molecular mechanism. Methods 100 New Zealand rabbits were randomly divided into normal group, model group, sham operation group, BYS group, Western medicine group and 20 in each group, and establish CRS model after seven days, feed. BYS group were given BYS 1.5mL· kg-1·d-1, Western medicine group given Clarithromycin 25mg·kg-1 · d-1, altogether 14 days, put to death after taking nasal sinuses mucosa tissue, observe nasal sinuses mucosa pathological changes with light microscopy after HE dyeing, de- tect nasal sinuses mucosal epithelium NF - κB DNA activity with gel electrophoretic migration analytical method, and IL -1β mRNA expression with RT - RCR. Results Model group appeared chronic inflammation and inflammatory cell infiltration, epithelial cells and glandular organs and goblet cells were hyperplasia obviously. NF-κB DNA activity and IL -1β mRNA expression were higher than normal group（ P 〈 0.01 ）. After the treatment of BYS the nasal sinuses mucosa epithele repaired better, inflam- matory cells and glandular organs and goblet cells were not hyperplasia obviously. NF -κB DNA activity and IL-1β mRNA expression are lower than model group obviously （ P 〈 0. 05 ）, and had no differences with nomal group （ P 〉 0. 05 ）. Conclusion BYS can suppress NF-κB DNA activity and IL-1β genetic transcription possibly.