3种不同方法在小儿肺炎支原体检测中的价值比较

Comparison in detection value of three different methods in pediatric pneumonia mycoplasma

目的探讨3种不同方法在小儿肺炎支原体检测中的应用效果。方法选择深圳市南山区西丽人民医院收治的1 800例呼吸道感染患儿为研究对象,分别用间接免疫荧光法、被动颗粒凝集法、培养法检测支原体感染,并比较3种检测方法的阳性率、灵敏度、特异性。结果培养法阳性率与间接免疫荧光法及被动颗粒凝集法相比,差异无统计学意义(P>0.05);间接免疫荧光法与被动颗粒凝集法阳性率差异也无统计学意义(P>0.05);间接免疫荧光法与被动颗粒凝集法、培养法灵敏度、特异性经统计学分析,差异无统计学意义(P>0.05)。结论临床医师需根据病原体检测的优缺点,选择合适的方法,综合各种检测结果分析诊断肺炎支原体感染。

Objective To study the clinical effect of the three different methods in the infantile pneumonia my-coplasma detection .Methods 1 800 cases of children with respiratory tract infection were choosed as the research ob-ject .indirect immunofluorescence method ,passive particle agglutination and culture method were used to detect myco-plasma pneumonia .Positive rate ,sensitivity and specificity of three methods were observed .Results PCR positive rate was significantly higher than the indirect immunofluorescence method and passive particle agglutination method , the difference was statistically significant （P〈0 .05） .Indirect immunofluorescence method with the method of passive particle agglutination positive rate difference has no statistical significance （P〉0 .05） .Indirect immunofluorescence and passive particle agglutination method ,PCR method ,the sensitivity ,specificity ,after statistics analysis ,there was no significant difference（P〉0 .05） .Conclusion Clinicians should be based on the advantages and disadvantages of pathogen detection ,choosing the appropriate method ,based on the analysis of all kinds of test results in the diagnosis of mycoplasma pneumoniae infection .