UF-1000i尿沉渣分析仪与显微镜检测尿液小圆上皮细胞对比分析及参考值建立

UF-1000i urine sediment analyzer and microscope detection small round epithelial cells results contrast analysis and establish the reference range

目的通过对UF-1000i尿沉渣分析仪与显微镜检测尿液中小圆上皮细胞结果对比分析及其参考值的建立,为临床提供更为准确可靠的实验数据和诊断参考区间。方法随机收集沙井人民医院肾内科及泌尿外科住院或门诊患者165例中段清晨尿,分别用UF-1000i尿沉渣分析仪与显微镜检测尿液中小圆上皮细胞,对检测结果进行对比分析。同时随机收集1 296例健康体检者中段清晨尿液用显微镜检测尿中小圆上皮细胞,建立健康人群的临床诊断参考区间。结果以显微镜检测为金标准,尿液中白细胞正常情况下,UF-1000i尿沉渣仪检测尿液小圆上皮细胞略高于显微镜法,但差异无统计学意义(t=1.24,P>0.05),而尿液中白细胞增高情况下,UF-1000i检测结果高于显微镜法,差异有统计学意义(t=6.92,P<0.01)。健康人群中段晨尿小圆上皮细胞参考值,年龄与性别之间差异无统计学意义(t=1.06,P>0.05),但女性结果略高于男性。结论尿液中白细胞增高情况下,UF-1000i尿沉渣分析仪检测小圆上皮细胞与显微镜检测结果之间有较大的差异,对白细胞检查结果异常者需用显微镜进行复检,以提高结果的准确性;建立生化指标参考区间,为临床诊断、治疗提供更有价值的诊断依据。

Objective U F-1000i urine sediment analyzer and microscope detection small round epithelial cells results contrast analysis and the reference range ,provide a more accurate and reliable experimental data for clinical and scientific diagnostic reference range .Methods 165 cases of urine specimen of midway through the morning urine were collected in urology and small and medium-sized round epithelial cells from those specimen were detected by UF-1000i or under microscope respectively .While 1 296 cases randomly collected health check-up crowd midway through the morning urine microscope detection small round epithelial cells ,establish healthy crowd reference range of clinical diagnosis of urinary small round epithelial cells .Results With microscope examination as the gold stand-ard ,white blood cells in the urine under normal circumstances ,UF-1000i urine sediment to test urine small round epi-thelial cells slightly higher than the microscopy ,there was no statistically significant difference between the results （t=1 .24 ,P〉0 .05） ,and white blood cells in urine increased cases ,UF-1000i test results than microscopy ,the results have significant difference between statistical significance （t= 6 .92 ,P〈 0 .01） .Healthy crowd midway through the morning urine small round epithelial cells biology reference range ,there was no statistically significant difference be-tween age and gender（t=1 .06 ,P〉1 .06） ,the female results slightly higher than the male .Conclusion Urine leuco-cyte heighten ,UF-1000i urinary sediment analyzer to detect urinary small round epithelial cells has bigger difference between test results and microscope ,the leukocyte results abnormal urinary epithelial cells of small round epithelial cells inspections with microscope ,in order to improve the accuracy of the results .Establish biological reference range , provide more valuable for clinical diagnosis ,treatment of diagnosis .