摘要
目的探讨慢性肾衰竭患者血清血管细胞黏附分子-1(VCAM-1)与血管性假性血友病因子(vWF)的变化及临床意义,以及血液透析对其水平的影响。方法应用酶联免疫吸附试验(ELASA)测定30例慢性肾衰竭未透析患者(CRF组)、30例血液透析患者(HD组)、30例健康自愿者(健康对照组)血清VCAM-1与vWF的水平,并比较透析前后的变化。结果 VCAM-1水平在HD组、CRF组及健康对照组分别为(7 638.4±372.2)ng/mL、(6 723.5±136.4)ng/mL、(2 901.3±253.2)ng/mL,HD组、CRF组明显高于健康对照组,差异均有统计学意义(P<0.01,P<0.01)。vWF水平在HD组、CRF组以及健康对照组分别为(241.7±13.5)%、(165.6±7.9)%、(90.5±l0.8)%,HD组、CRF组明显高于健康对照组,差异有统计学意义(P<0.01,P<0.05)。血清VCAM-1透析后(7 863.4±186.3)ng/mL较透析前(7 638.4±372.2)ng/mL明显升高,差异有统计学意义(P<0.05)。vWF透析后(291.3±12.6)%较透析前(241.7±13.5)%明显升高,差异有统计学意义(P<0.05)。结论慢性肾功能衰竭患者及维持性血液透析患者存在血管内皮功能紊乱,血液透析可进一步加重其损害。
Objective To investigate the change of plasma VCAM-1 and vWF in patients with chronic renal failure and their clinical significance ,and the influence of hemodialysis .Methods The levels of plasma VCAM-1 and vWF were measured in CRF patients without hemodialysis （CRF group ,n=30） ,hemodialysis patients（HD group ,n=30）and control group（n=30） by the method of enzyme linked immunosorbent assay （ELISA） .Results The levels of plasma VCAM-1 in HD group were higher than CRF group ,both the two groups were significantly higher than the control group ,the value of three groups respectively were （7 638 .4 ＆#177; 372 .2）ng/mL ,（6 723 .5 ＆#177; 136 .4）ng/mL , （2 901 .3 ＆#177; 253 .2）ng/mL（P〈0 .05） .The value of vWF in HD group and CRF group were（241 .7 ＆#177; 13 .5）% ,（165 .6 ＆#177; 7 .9）% ,significantly higher than the control group（90 .5 ＆#177; 10 .8）% （P〈0 .05） .The levels of plasma VCAM-1 and vWF of pre-HD group and post-HD group had significantly difference ,post-HD group were higher than pre-HD group（P〈0 .05） .Conclusion There was endothelial dysfunction in patients with chronic renal failure and mainte-nance hemodialysis ,the process of hemodialysis may contribute to endothelial dysfunction .
出处
《检验医学与临床》
CAS
2014年第12期1648-1649,1652,共3页
Laboratory Medicine and Clinic
