‘扎西玛’与‘南粳46’籼粳杂交F_1花药培养及再生体系的建立

Anther Culture of Hybrid F_1 of Indicaand Japonica Varieties ‘Zaxima'/‘Nanjing 46'

为探索籼粳杂交水稻花药培养再生体系,以籼稻‘扎西玛’和粳稻‘南粳46’籼粳杂交F1花药为外植体,分别从低温预处理时间、基本诱导培养基种类、蔗糖质量浓度及不同激素配比方面研究花药愈伤组织诱导,并将诱导出的愈伤组织进行分化成苗,以期构建籼粳地理远源杂交水稻花药培养再生体系。结果表明,4℃处理2-5d的花药,其愈伤组织诱导率较高,与未处理和低温处理6-10d相比达到显著水平;添加蔗糖质量浓度为60g·L^-1,以N6或M8为基本培养基均可以获得较高的诱导率;以M8为基本培养基,添加2,4-D 2mg·L^-1和KT 0.3mg·L^-1诱导水稻愈伤组织出愈率可高达32.14%;以MS为基本培养基,激素组合为KT 2mg·L^-1和ABA 3mg·L^-1分化培养基,其愈伤组织成苗率可达50%,其中绿苗分化率达4.55%。

To establish an efficient regeneration system of hybrid F1of Indicaand Japonicavarieties,anthers of F1from crosses of Indicaand Japonicavarieties‘Zaxima＇/‘Nanjing 46＇were used as the explants to induce the callus in different low-temperature pretreatment time,different basic media,different media supplemented with different mass concentrations and combinations of sucrose and plant hormones.The result showed anthers pretreated at 4 ℃for 2-5days were induced to callus in a higher level,significantly higher than those under other pretreatments.Medium N6and M8were better than the SK3medium in callus induction.Callus induction frequency was significantly different,and the incorporation of sucrose in the mass concentration of 60g·L^-1 in the callus induction medium.The 32.14%of callus inducting rate in the M8medium added with 2,4-D 2mg·L^-1＋ KT0.3mg·L^-1 was obtained and the 50% of callus differentiation rate in the MS medium added with KT 2mg·L^-1＋ABA 2mg·L^-1 was obtained,but the frequency of green plantlet was almost4.55%.