转大肠杆菌KatG基因烟草的获得及其抗逆性鉴定

Gain of the KatG Genetic Tobacco and Analysis on Stress Resistance of Transgenic Tobacco

以NC89烟草(N.tabacum)为材料,经农杆菌介导法转化烟草,通过PCR和RT-PCR对转化烟草进行鉴定,利用百草枯、PEG6000和NaCl处理烟草并进行抗性分析。结果表明,经不同浓度百草枯处理后,转基因烟草受伤害程度明显低于野生型,说明转KatG烟草的抗氧化性强于野生型烟草。与野生型植株相比,经不同质量浓度PEG6000和不同浓度NaCl处理后,转基因烟草叶片相对含水量、相对电导率、CAT活性、MDA质量摩尔浓度、PSⅡ相对量子产率均显著优于野生型植株,表明转KatG烟草的抗旱和抗盐性强于野生型烟草。说明大肠杆菌KatG基因具有提高植物抗逆的功能。因此,KatG基因在抗逆基因工程方面具有较好地应用前景,大肠杆菌KatG基因具有增强抗氧化的功能。

NC89tobacco plants were served as material to transform the foreign gene KatG via Agrobacterium tumefaciens.PCR and RT-PCR indicated that KatGgene had been integrated into the genome of the tobacco.The transgenic tobacco plants were tested tolerance to paraquat,PEG6000and NaCl.The results indicated that with the treatments of different concentration of paraquat,leaf injury of transgenic tobacco was significantly lower than wild type plants（WT）,which suggested the transgenic tobaccos had higher ability of anti-oxidation than WT.The physiological indexes of KatGtobacco,including leaf relative water content,relative conductivity,CAT activity,molality of solute of MDA and maxi-mum photochemical yield of PSⅡ,were evidently higher than those of WT under the stress of different concentration of PEG6000and NaCl.Thus,the transgenic plants had more tolerance to stress.Therefore,catalase gene KatGof Escherichia coli have the function of increasing antioxidation,and KatGgene had a better application prospect on the stress-tolerance genetic engineering.