4.10MPa氢氧暴露对大鼠氧化应激影响研究

Study on the effect of oxidative stress in rats exposed to 4.10MPa H_2-O_2

目的探讨4.10 MPa氢氧暴露对大鼠氧化应激的影响。方法无特定病原体级健康成年雄性SD大鼠30只随机分为对照组、氦氧组和氢氧组。3组大鼠分别在动物加压舱内的常压常氧空气、4.10 MPa氦氧及4.10 MPa氢氧环境中暴露24 h。出舱后处死大鼠,分离脑、肺和肝组织,比色法测定超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力和还原型谷胱甘肽(GSH)、丙二醛(MDA)和8-羟基脱氧鸟苷(8-OhdG)水平。结果对照组、氦氧组和氢氧组3组大鼠脑组织SOD、GSH-Px活力及GSH水平分别比较,差异均有统计学意义[(153.09±37.62)vs(114.97±23.38)vs(162.31±36.03)mmol/(min·gprot),(1 042.27±248.32)vs(746.77±188.13)vs(1 040.73±192.41)μmol/(min·gprot),(2.45±0.64)vs(1.46±0.36)vs(2.06±0.49)mg/gprot,P<0.05,P<0.05,P<0.01];氦氧组脑组织SOD、GSH-Px活力及GSH水平均低于对照组(P<0.05),氢氧组脑组织SOD、GSH-Px活力和GSH水平均高于氦氧组(P<0.05)。3组大鼠肺组织GSH-Px活力和GSH水平分别比较,差异均有统计学意义[(437.29±46.62)vs(358.34±31.49)vs(446.40±55.52)μmol/(min·gprot),(2.30±0.55)vs(0.98±0.13)vs(1.67±0.30)mg/gprot,P<0.01,P<0.05];氦氧组肺组织GSH-Px活力和GSH水平均低于对照组(P<0.05),氢氧组肺组织GSH-Px活力高于氦氧组(P<0.05)。3组大鼠肝组织SOD和GSH-Px活力分别比较,差异均有统计学意义[(101.31±9.67)vs(93.99±6.13)vs(112.23±11.41)mmol/(min·gprot),(325.28±34.39)vs(276.67±22.29)vs(341.05±31.74)μmol/(min·gprot),P<0.01];氦氧组肝组织GSH-Px活力低于对照组(P<0.05),氢氧组肝组织中SOD和GSH-Px活力均高于氦氧组(P<0.05)。氢氧组脑、肺和肝3种组织SOD、GSH-Px活力及GSH水平分别与对照组相应组织比较,差异均无统计学意义(P>0.05)。3组组间脑、肺和肝3种组织的MDA及8-OhdG水平分别比较,差异均无统计学意义(P>0.05)。结论 4.10 MPa氢氧暴露大鼠的氧化应激指标未显现出明显改变,与氢对高氧毒性的抑制作用有关。从氧化应激的角度看,高压氢氧环境比高压氦氧环境具有更好的生理安全性。

Objective To investigate the effect of oxidative stress in rats exposed to 4.10 MPa H2-O2 .Methods Totally thirty male SD rats were randomly divided into a control group , a He-O2 group and a H2-O2 group, which were exposed re-spectively to normbaric normoxic air , 4.10 MPa He-O2 and 4.10 MPa H2-O2 for 24 hours with an animal chamber .Rats were sacrificed after exposure .Brain, lung and liver tissues were obtained at autopsy for the following tests .The activities of superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px） and levels of reduced glutathione （GSH）, malondial-dehyde （MDA）, 8-hydroxy-2＆#39;-deoxyguanosine （8-OhdG） were determined by colorimetric methods .Results The diffe-rences of brain SOD, GSH-Px and GSH among the control , He-O2 and H2-O2 groups were statistically significant respectively [（153.09 ±37.62） vs （114.97 ±23.38） vs （162.31 ±36.03） mmol/（min· gprot）, （1 042.27 ±248.32） vs （746.77 ±188.13） vs （1 040.73 ±192.41） μmol/（min· gprot）, （2.45 ±0.64） vs （1.46 ±0.36） vs （2.06 ± 0.49） mg/gprot, P〈0.05, P〈0.05, P〈0.01].Brain SOD, GSH-Px and GSH in He-O2 group were lower than those in control group respectively （P〈0.05）.Brain SOD, GSH-Px and GSH in H2-O2 group were higher than those in He-O2 group respectively （ P〈0.05 ） .The differences of lung GSH-Px and GSH among 3 groups were statistically significant＆amp;nbsp;respectively [（437.29 ±46.62） vs （358.34 ±31.49） vs （446.40 ±55.52） μmol/（min· gprot）, （2.30 ±0.55） vs （0.98 ±0.13） vs （1.67 ±0.30） mg/gprot, P〈0.01, P〈0.05].Lung GSH-Px and GSH in He-O2 group were lower than those in control group respectively （P〈0.05）.Lung GSH-Px in H2-O2 group were higher than those in He-O2 group （P〈0.05）.The differences of liver SOD and GSH-Px among 3 groups were statistically significant [（101.31 ±9.67） vs （93.99 ±6.13） vs （112.23 ±11.41） mmol/（min· gprot）, （325.28 ±34.39） vs （276.67 ±22.29） vs （341.05 ± 31.74） μmol/（min· gprot）, P〈0.01].Liver GSH-Px in He-O2 group was lower than that in control group （P〈0.05）. Liver SOD and GSH-Px in H2-O2 group were higher than those in He-O2 group respectively （P〈0.05）.SOD, GSH-Px and GSH of brain, lung, and liver in H2-O2 group showed no statistical significant differences （P〉0.05）, when compared with the control group .There were no statistical significant differences in MDA and 8-OhdG of brain, lung, and liver among 3 groups （P〉0.05）.Conclusion No obvious changes were shown in oxidative stress of 4.10 MPa H2-O2 exposed rats, which was correlated with attenuation of hyperoxic toxicity by hydrogen .The data suggests that hyperbaric H 2-O2 envi-ronment may be physiologically safer than hyperbaric He-O2 environment from perspective of oxidative stress .