MCAO模型大鼠中医证候研究

Syndrome research of MCAO model rat

目的:探讨大脑中动脉栓塞(middle cerebral artery occlusion,MCAO)模型大鼠中医证候。方法:SD大鼠70只随机分为正常组、假手术组、手术组,假手术组与手术组按不同时间分为6 h、48 h、72 h组,每组10只。以大脑中动脉栓塞法建立MCAO大鼠模型,分别在手术完成后6 h、48 h、72 h时观察大鼠的一般状况、Zea-longa神经功能评分、放射免疫法检测大鼠血清皮质酮(cortisone,CORT)水平,光镜下观察大鼠脑组织形态结构变化。结果:模型大鼠偏瘫症状明显,行走呈顺钟向追尾状,甚则向一侧倾倒;精神萎靡、活动减少、身体蜷缩、体质量明显下降、舌质紫暗、神经功能Zea-longa评分明显增高,血清CORT水平明显增高,脑组织形态结构发生明显改变。结论:MCAO大鼠存在明显的肾虚血瘀证证候。

Objective： To study the TCM syndrome of middle cerebral artery occlusion（ MCAO） model rat; Methods：Seventy SD rats were randomly divided into normal group,sham operation（ sham） group and operation group. The sham and operation groups according to different time were divided into 6 h,48 h and 72 h groups. Ten rats in every group. To establish MCAO rats model with the method of MCAO. At the time points of 6 h,48 h and 72 h after the operation,the general conditions and the Zea-longa nerve function score were observed. The concentration of cortisone（ CORT） in serum was detected with radioimmunoassay. The morphological structure of cerebral tissue was observed with light microscope.Results： The model rats walked with hemiplegia symptoms,dumping to one side and walked along the clock. It also showed curl,listlessness and decreased activity. The weight decreased obviously. Its tongue was dark purple. Zea-longa nerve function score significantly increased. The level of CORT in serum of MCAO model rat increased significantly. The morphology structure of brain tissue changed greatly. Conclusion： MCAO rat shows TCM syndrome with kidney deficiency and blood stasis.