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鼠疟模型作为恶性疟原虫多表位疫苗保护性的探索试验 被引量：1

PRELIMINARY STUDIES ON MOUSE PLASMODIUM YOELII MODEL FOR THE EVALUATION OF A MULTI EPITOPE VACCINE CANDIDATE OF PLASMODIUM FALCIPARUM
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摘要 目的 :为寻找恶性疟原虫基因工程多表位疫苗保护性评价的模型动物 ,利用小鼠约氏疟原虫 ( Plas-modium yoelii)及小鼠伯氏疟原虫 ( Plasmodium berghei)模型对本实验室构建的恶性疟原虫 ( Plasmodiumfalciparum)多阶段、多表位基因工程候选疫苗进行了体内保护性及免疫模式探索试验。结果在约氏疟原虫模型 ,先用该疫苗基因的大肠杆菌表达蛋白免疫 ,后用含同一基因的重组痘苗病毒加强免疫组 ,与野生型痘苗病毒免疫组及空白对照组相比 ,小鼠的死亡时间延长 ( P<0 .0 1)。结果初步显示 。 To find a suitable animal model for the vaccine assessment of Plasmodium falciparum,Plasmodium yoelii and Plasmodium berghei of mouse were used to evaluate the protection for a multi epitope vaccine candidate of Plasmodium falciparum . Results showed that mice of the group firstly immunized with the protein expressed in E.coli ,then boosted with the recombinant vaccinia virus from the same epitopes survived longer than that of groups immunized with the wild vaccinia virus or the blank control ( P <0.01).This indicates that Plasmodium yoelii of mouse could be used as a protective model for the evaluation of multi epitope vaccine candidate of Plasmodium falciparum .
作者 董文其 毕惠祥 李明 曲利芝
机构地区 第一军医大学热带医学研究所 第一军医大学实验动物中心
出处 《寄生虫与医学昆虫学报》 CAS 2001年第1期1-6,共6页 Acta Parasitologica et Medica Entomologica Sinica
基金 UNDP/WH O Special Programme for Research and Training in Tropical Disease ( TDR)的资助
关键词 恶性疟原虫 约氏疟原虫 疫苗 鼠疟模型 Plasmodium falciparum Plasmodium yoelii Vaccine
分类号 R392-33 [医药卫生—免疫学]
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寄生虫与医学昆虫学报
2001年 第1期

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