一株产微生物絮凝剂菌株的分离鉴定及特性

THE SCREEN AND CHARACTERIZATION OF A FLOCCULANT-PRODUCING BACTERIUM

从活性污泥中分离筛选到一株产絮凝剂的细菌 A2 5,鉴定为巨大芽孢杆菌 Bacillusmegaterium。该菌株产絮凝剂的最适碳源为麦芽糖 ,最适氮源为酵母提取物 ,最适 p H为 7.0～ 10 .0。絮凝剂的形成与菌体生长同步 ,均在 10 h达到最高值。该絮凝剂主要分布在发酵液中 ,另外还有一部分存在于菌体上。所产絮凝剂对供试的各种悬浮液和菌悬液都具有良好的絮凝效果。

Abacterial strain A25, isolated from activated sludge and identified as Bacillius megaterium,could produce flocculant.The optimum carbon and nitrogen sources are maltose and yeast extract, respectively.The optimum pH range is 7.0~10.0. The flocculant was synthesized during the cell growth.The flocculant mainly exists in the supermatant.The partial purified flocculant could be kept in 60℃ for 1 hour without losing activity.