海洋真菌多不饱和脂肪酸的快速检测

A FAST DETERMINATION OF POLYUNSATURATED FATTY ACIDS PRODUCED BY MARINE FUNGUS

从海水中分离了一种产 EPA和 DHA的真菌 ,并建立了微生物油脂的快速提取、甲酯化及其多不饱和脂肪酸的含量的气相色谱测定方法。结果表明 :采用 CHCl3 - KOH- CH3 OH一步提取甲酯化适合于产多不饱和脂肪酸菌株的快速检测。对其油脂的提取 ,采用湿菌丝魏氏盐酸水解法较为合适 ;并用 KOH-CH3 OH法对所得油脂进行了甲酯化。最后 ,运用毛细管气相色谱采用外标法测定了甲酯化样品中多不饱和脂肪酸的含量 ,其油脂中 EPA和 DHA的含量分别为 7.32 %和 7.58%。

A fungus that is capable of producing EPA and DHA was isolated from seawater. A fast extraction and methyl esterification of microbial lipid were established as well as their content determination by capillary gas chromatography using external calibration method. The results showed that the methyl ester prepared directly form the wet mycelia treated with CHCl 3 KOH CH 3OH is less time consuming and the method fits for the fast determination. To determine its EPA and DHA content,the wet mycelia was hydrolyzed according to Weibul’s method and the lipid obtained was methyl esterified with KOH MeOH,the results obtained by capillary gas chromatography using external calibration method were 7.32% and 7.58% respectively.