黑石顶南亚热带常绿阔叶林优势种群黄果厚壳桂的AFLP分析

AFLP analysis in Cryptocarya concinna,a dominant population in lower subtropical evergreen broad-leaved forests at Heishiding Natural Reserve

以扩增片段长度多态性 ( AFLP)分析了黑石顶南亚热带常绿阔叶林优势种群黄果厚壳桂 ( Cryptocarya concinna)在三个不同群落类型中的遗传多态性及遗传结构 ,并比较了该 3个种群间的遗传分化。以 4对 AFLP选择引物组合产生了大量扩增表型带 ,用 AMOVA、PHYLIP等软件分析计算了种群的遗传结构。结果表明黑石顶黄果厚壳桂种群具有高的种群内遗传多态性、低的种群间遗传分化。并讨论了种群遗传变化与生境的关系。

Amplification fragment length polymorphism (AFLP) was employed to analyze population genetic structure and variation of Cryptocarya concinna, a dominant species in lower subtropical evergreen broad leaved forests.37 individuals of Cryptocarya concinna from 3 different communities in Heishiding Natural Reserve were sampled and their DNAs were amplified using 4 AFLP selective primer combinations.51 polymorphic phenotypic patterns (loci) were calculated and analyzed using Shannon information coefficient,AMOVA and PHYLIP for the genetic diversity and differentiation of the 3 Cryptocarya concinna populations.High genetic diversity with the population and low differentiation among the 3 populations were detected based on SSD (Sum of squared deviations), MSD (Mean squared deviations) and variance components of AMOVA statistic analysis.These were consistent with the results from Shannon coefficient calculation.Nei's genetic distance also indicated very low differentiation among the populations.The relationship of population genetic variation and the microhabitat was discussed.