汉坦病毒感染诱导乳鼠脑神经细胞表达热休克蛋白70

HSP72 like Immunoreactivities in the Brains of Mice Experimentally Infected with Hantavirus

生后 2～ 3天的昆明乳鼠 ,每只腹腔接种 0 .0 5mL 10 0个半数致死量的陈株汉坦病毒 ,于接种后不同时间点处死动物 ,取其脑组织常规固定 ,石蜡包埋制备 5 μm连续组织切片 ,用免疫组化法检测组织中的病毒抗原及热休克蛋白 70的表达 ,用共聚焦显微镜观察二者之间的关系。结果发现 ,感染了病毒的组织能够稳定地检测到热休克蛋白 70的表达 ,而病毒阴性的组织则检测不到热休克蛋白 70的表达 ,且二者有共定位关系 ,其分布与组织病变一致。这与在病毒感染的VeroE6细胞及EHF病人尸检组织中得到的结果一致 ,说明病毒感染可诱导热休克蛋白 70的表达 。

Sucking mice, 2～3 day after birth, were intraperitoneally inoculated with 0.05mL F 1M 10 of Chen Strain Hantaan virus. At different time point after inoculation, the brains were taken, routinely fixed and embedded in paraffin for preparing 5 μm serial sections. Traditional and confocal immunochemical detection of viral antigens and heat shock protein 70 were performed to explore the cerebral stress response after viral infection. The results showed that HSP70 immunoreactivities could be stably detected in the viral antigen positive neurons, but not in the viral antigen negative or uninfected control tissues. By confocal microscopic examination, the HSP70 and viral antigens were colocolized in the neuronal cytoplasm. Our result, comparable to our previous findings in human tissues and culture cells, indicated that Hantavirus infection can induce the expression of HSP70 in the infected cells, and HSP70 expression might be necessary but not sufficient to keep the cell survival.