从云南省蝙蝠脑组织中分离出乙型脑炎病毒

Isolation of Japanese Encephalitis Virus from Brain Tissues of Bat in Yunnan Province

为进一步阐明蝙蝠在保存乙脑病毒中的作用 ,于 1997年 7月 ,在云南省耿马县捕捉蝙蝠 6 4只 ,取脑组织作病毒分离 ,从一只金管鼻蝠脑组织中分离出 1株病毒。该毒株能引起BHK2 1细胞病变和乳鼠发病死亡 ,在 pH5 75～7 4时能凝集鸽红血球 ,经用单克隆抗体血凝抑制和免疫荧光试验鉴定 ,证实为乙型脑炎病毒。进一步证明蝙蝠在乙型脑炎病毒保存和扩散中具有重要作用。

In July 1997, a strain (GB30) of virus was isolated from 60 samples of brain tissues of Murina aurata (Chiroptera: Vespertilionidae) collected in Gengma county, Yunnan province, China. Isolation of virus was negative from 4 samples of brain tissues of Rousettus leschenaulti (Chiroptera: Pteropodidae) collected in Gengma. GB30 virus strain could regularly cause illness and death in suckling mice, produced evident CPE in BHK21 cells. It agglutinated red blood cells of dove at pH5.75～7.4. This virus has been identified serologically by hemagglutination inhibition and immunofluorescent tests using Japanese encephalitis (JE), dengue (DEN) type 1,2,3,4, and chikungunya (CHIK) viruses monoclonal antibodies, and JE and sindbis (SIN) viruses immune sera. It showed specific reaction to JE virus only and no reaction with DEN 1～4, CHIK and SIN viruses. Therefore it can be identified as JE virus. This is the first report on the isolation of JE virus from Murina aurata . The results showed that bats are considered as the reservoir and amplifier host of JE virus transmission in nature.