医用钛合金表面聚电解质层接枝酪蛋白磷酸肽复合薄膜的生物矿化评价

Evaluation of the Biomineralization of PEC Film Graft CPP on Medical Titanium Alloy

采用层层自组装的方法,在羟基化和硅烷偶联剂处理的医用钛合金表面交替组装海藻酸钠和壳聚糖,再通过酰胺化反应接枝酪蛋白磷酸肽,在医用钛合金表面形成具有仿生矿化能力的聚电解质复合薄膜。采用XPS、ATR-FTIR、FESEM以及XRD对样品表面进行表征,并将各组样品放入模拟体液中考察仿生矿化沉积能力。结果表明:在医用钛合金表面成功制备了聚电解质层接枝酪蛋白磷酸肽复合薄膜,经仿生矿化的各组样品表面均有羟基磷灰石沉积,聚电解质层组表面具有纳米多孔结构以及羧基和羟基活性基团,接枝酪蛋白磷酸肽后表面又具有磷酸活性基团,因此最终形成的复合薄膜羟基磷灰石沉积最为致密,表明其生物矿化能力明显增强。

The Ti alloy for medical appliancations were firstly hydroxylated and modified by silane coupling agents, then the alginate and chitosan were fabricated by layer-by-layer self-assembly, finally casein phosphopeptide was grafted via amidation reaction, so the CPP-grafted polyelectrolyte complex film was prepared on the medical Ti al- loy surfaces. The samples were mineralized in simulated body fluid, and the ability of biomineralization was characte- rized by XPS, ATR-FTIR, FESEM and XRD. The results showed that the Ti alloy surfaces were successfully modi- fied and the hydroxyapatites were deposited on the surface of every group. The group of polyelectrolyte layer had good nano-porous structure, and included responsable active groups such as the carboxyl group, the hydroxyl group, and the phosphate group grafted the casein phosphopeptide, therefore the polyelectrolyte complex film showed the dense of hydroxyapatite. All this suggested that the CPP-grafted polyelectrolyte complex film was of better capacity of biomi- neralization.