对虾白斑综合征杆状病毒同源性比较的研究

Homology Comparison of White Spot Syndrome Baculovirus (WSSV) from Penaeid shrimp

比较我国沿海不同海域对虾白斑综合征杆状病毒三个分离株 :即唐海分离株 (渤海湾 ) ,宁波分离株 (东海 ) ,深圳分离株 (南海 )的同源性。三个WSSV分离株基因组的限制性内切酶 (SacI ,HindIII,PstI)酶切多态 (RFLP)以及病毒结构蛋白图谱完全一致 ,证实造成我国从南至北对虾爆发性流行病的对虾白斑杆状病毒为同一种病毒。利用高保真Taq酶 ,分别以报道的日本对虾杆状病毒 (RV -PJ =PRDV) ,斑节对虾白斑综合征杆状病毒 (WSBV =PmNOBIII)基因组核酸片段特异性引物进行PCR扩增 ,结果均能从中国对虾白斑杆状病毒 (WSSV)基因组中扩增得到相应大小的PCR产物 ,扩增产物序列分析表明中国对虾白斑杆状病毒 (WSSV)与斑节对虾白斑综合征杆状病毒 (WSBV =PmNOBIII) ,日本对虾杆状病毒 (RV -PJ =PRDV)同源率分别为 10 0 %与 97% 。

s: Homology of three WSSV isolates, which were sampled from representative maritime space of China: Tanghai isolate (Bo Bay of China), Ningbo isolate (East China Sea), Shenzhen isolate (South China Sea) was compared. Both of the genome RFLP patterns and the characteristic structural proteins SDS PAGE electropherograms showed that they were quite same. It suggested that they were the same kind of WSSV virus that caused explosive epidemic diseases of shrimps (EEDS) throughout southern and northern China. The same large PCR products achieved when using the PCR primers from RV-PJ=PRDV ( P. japonicus , Japan) and WSBV=PmNOBIII( P.monodon Taiwan, China) respectively to amplify the genome from P.chinensis (Tanghai, China) with high fidelity Taq Polymerase. The sequence identities of WSSV from P. chinensis with those from RV-PJ=PRDV ( P.japonicus , Japan) and WSBV=PmNOBIII ( P.monodon Taiwan, China) are 97% and 100% respectively, the results provided additional evidence that WSSV reported in different parts of the Asian and Pacific regions maybe quite the same or just different variants of the same virus.