金鱼嗜水气单胞菌的分离鉴定及药敏试验

Identification and Drug Sensitivity Test of Pathogenic Aeromonas hydrophilafrom Goldfish Carassius auratus

自病死金鱼肝脏中分离到一株优势菌,对其进行感染试验、培养特性观察、生化特性鉴定及16SrRNA序列分析。试验结果表明,该分离菌株为嗜水气单胞菌,与已报道的嗜水气单胞菌的16S rRNA序列同源性>99.3%。用纸片扩散法进行药敏试验,试验结果显示,该分离株对四环素类、喹诺酮类、磺胺类、头孢呋新、头孢他啶、头孢吡肟等21种药物敏感,对青霉素G、氨苄西林、阿莫西林、头孢氨苄、林可霉素、麦迪霉素耐药。本次金鱼发病是由嗜水气单胞菌感染引起,可选用强力霉素、麦迪霉素、复方新诺明、磺胺甲基异恶唑、阿奇霉素、恩诺沙星、诺氟沙星等多种药物进行防治。

Aeromonas h ydrophila is one of the main pathogen of freshwater fish bacterial septicemia .The bacterium was isolated and identified from dead gold fish and drug sensitive tests were performed in order to provide references for the bacterial disease prevention and control in ornamental fish .A dominant bacteria strain was isolated from hepatopancreas of dead Carassius auratus and identified by artificial infection experiment ,cultural characteristics ,physical and chemical characters ,and 16S rRNA sequence analysis .The results showed that the strain was A .hydrophila .Homology of 16S rRNA of the isolated strain and other several A .hydrophila was more than 99 .3% .Drug sensitive test revealed that the isolated strain was highly sensitive to 21 kinds of drugs , including tetracyclines ,quinolones , sulfonamides , cefuroxime ,ceftazidimeand cefepime ,and resistant to penicillin G ,ampicillin ,amoxicillin ,cefalexin , lincomycin and medemycin . Results in this study showed that many antibiotics （such as doxycycline , midecamycin ,co‐trimoxazole ,sulfamethoxazole ,azithromycin ,enrofloxacin ,and norfloxaci） can be used to control and prevent bacterial disease caused by A .hydrophila in gold fish .