摘要
应用流式细胞术分析烟草细胞在交变应力作用下细胞周期的变化。用特制的强声波发生装置产生频率和强度可调的交变应力场 ,研究不同频率和强度的交变应力作用后烟草细胞周期的变化。实验结果表明 ,在交变应力作用下直接影响细胞或细胞分裂的同步化 ,促进S期的DNA合成 ,有助于细胞有丝分裂。声波频率在400Hz至800Hz,强度在90dB到110dB内 ,随频率和强度的增加 ,交变应力使S期细胞明显增加 ,但频率或强度过大 ,反而使S期细胞大大减少。
Flow cytometry was used to measure cell cycle parameters in tobacco cell suspensions acted by alternative stress. The field of alternative stress was generated through a strong sound filed system. It was found that the effects of stress on cells depended greatly on the intensity and frequency of stress. In a certain range of intensity and frequency, the stress caused a significant changes to cell cycle paraments and an increase of the proportion of cells in S phase was seen, while the stress with higher frequency or intensity may cause the proportion of cells in S phase decreased evidently.
出处
《生物物理学报》
CAS
CSCD
北大核心
2001年第1期195-198,共4页
Acta Biophysica Sinica
