两种泥鳅不同核质关系下LDH同工酶基因表达的研究

THE EXPRESSION OF LDH ISOZYMIC GENES IN MISGURNUS ANGUILLICAUDATUS AND PARAMISGURNUS DABRYANUS UNDER DIFFERENT RELATIONS BETWEEN NUCLEUS AND CYTOPLASM

采用聚丙烯酰胺不连续系统凝胶电泳方法分析了泥鳅和大鳞副泥鳅不同杂交组合不同核质关系下胚胎发育阶段（０－１４５ｈ）中乳酸脱氢酶（ＬＤＨ）同工酶的分化表达谱式，ＬＤＨ同工酶的基因表达随细胞质、细胞核不同及胚胎发育各个时期具有不同的个体发育谱式。以泥鳅卵子为细胞质的Ⅰ、Ⅱ两组，杂交组（Ⅱ）ＬＤＨ同工酶基因在受精后３ｍｉｎ至原肠中期雄核基因参与表达与调控，部分基因位点比本交组（Ⅰ）启动与表达的时间要早，两组的管家酶主要来自细胞质。以大鳞副泥鳅为细胞质的Ⅲ、Ⅳ两组，各酶均以细胞质调控为主，其管家酶与泥鳅有很大不同。并具体分析和讨论了不同核质关系下ＬＤＨ同工酶基因的表达和调控的时空顺序。

The expression patterns and regulations of LDH isozymic systemare investigated in the early developmental stages [from unfertilized eggs to the one chamber air bladder stage (0-145h)] in different combinations between nucleus and cytoplasm of M. anguillicaudatus and P. dabryanus. Isozymes are resolved by polyacrylamide gel electrophoresis and then detected by using specific histochemical staining. The expression patterns and regulations of LDH isozymic systems are different in the different combinations of nucleus and cytoplasm. Group Ⅰ and Ⅱ had the same cytoplasm of M. anguillicaudatus, and the male nucleus genes of LDH are expressed in hybridization group (Ⅱ) from 3min of post-fertilization to mid gastrula stage. However their gene products in inbreeding group (Ⅰ) are not expressed. Some of the isozymic genes in hybridization group are expressed earlier than in inbreeding group. The house keeping enzymes of group Ⅰ and Ⅱ are from cytoplasm. Group Ⅲ and Ⅳ have the same cytoplasm of P. dabryanus, the LDH isozymic genes are expressed and regulated by cytoplasm, their house keeping enzymes are very different from those of M. anguillicaudatus. The temporal and spatial expressions and regulations of LDH genes in different combinations between nucleus and cytoplasm are discussed.