双歧杆菌的辐照育种研究

STUDIES ON ^( 60 ) CO RADIATION SELECTIVE STRAINS OF BIFIDOBACTERIUM

对生产活菌微生态制剂的双歧杆菌东方 10 1号 ( df10 1)菌株进行了 6 0 CO辐照育种研究。通过 40 0 0～ 6 0 0 0 rad钴原 2次 2 4h循环辐照 ,选育出 df10 4和 df10 6两个菌株。鉴定结果表明 ,该两个菌株除在生化反应上略有改变外 ,其他特性未见任何改变。但选育株在液态培养物中 ,活存时间与存活率较原株有非常显著的提高。其培养物在室温 ( 2 0～ 37℃ )保存 2 7个月 ,df10 4与 df10 6尚有 5 .47× 10 5 CFU /m l和6 .78× 10 5 CFU/m l活菌。而在 6～ 8℃冰箱保存 ,则存活率仍达 36 .6 4%和 48.31%。此为生产活菌微生态制剂 (特别是液态型制剂 )

Study on 60 Co radiation selective strains of Bifidobacterium df101 for production of viable bacteria microecological products Cyclic radiation of two times 24 hours by use of 4 000～6 000 rad 60 Co balt source Two strains of df104 and df106 are selected come out Identified results:Two strains has micro-modification except for in biochemical reaction,other character there are not change But df104and df106 strains has highest live rate then df101 in liquid cultures Liquid cultures of df104 and df106 on the room temperature(20 to 37℃)store 27 months,still keep had 5 47×10 5 cfu/ml and 6 78×10 5 cfu/ml viabil bifidobacteria If grants its put on refrigerctor(6 to 8℃)them live rate and 36 64%(df104)and 48 31%(df106)in after two years plus three months Therefore,it may be provided best strains for production microecological products (especial in liquid from microecological products) of viabil bifidobacteria