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生物发光法测定溶脲脲原体内微量ATP

MEASUREMENT OF INTRACELLULAR MICRO-ATP OF UREAPLASME UREALYTICUM BY BIOLUMINESCENCE
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摘要 目的 监测在液体培养基中生长的溶脲脲原体 ( U u)细胞内微量 ATP的变化趋势 ,方法 运用生物化学发光技术 ,建立 ATP定量法。结果  Uu标准血清型 1( U1 )在孵育后的 3~ 12 h内其 ATP曲线呈活跃的上升趋势 ,且不受青霉素的影响 ,12 h后培养液内 ATP的总量已达到为 3.15 41× 10 - 1 1 mol,接近预期的标准。结论  U1 的 ATP曲线在质控范围内 ,培养 12小时后 ,其生长速度已接近对数生长期。因此 ,3~ 12小时这一时段适合观察抗生素对 Uu生长的影响 ,从而为体外客观而快速地进行 Developed a ATP micro-quantitative system based on bioluminescence,which can monitor the growth of broth-cultured ureaplasma urealyticum(Uu)indicated by ATP fluctuation The result showed that ATP curve of Uu standard serovar-1(U 1)ascended actively without the inhibition of penicillin during the 3~12 hours of incubation Comparison with the maximum ATP synthesis of Uu,3 1541×10 -11 mol ATP after 12 hours of U 1 culture suggests the exponential growth phase will coming. In conclusion,the 3~12 h ATP biolumine-scence system is well controllable,which is eminently suited for the observation of antibiotic influence on the Uu growth and further evaluation of thd Uu susceptibility in vitro
出处 《中国微生态学杂志》 CAS CSCD 2001年第1期22-24,共3页 Chinese Journal of Microecology
基金 中华医学会皮肤科学会科研基金资助
关键词 溶脲脲原体 腺苷三磷酸 生物发光法 ATP ureaplasma urealyticum adenosine triphosphate(ATP) bioluminescence
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  • 1Stanley P E,Anal Biochem,1969年,29卷,381页
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