摘要
The full\|length cDNA of mosquito esterase B1 had been isolated,and subcloned into pBV220.The recombinant vector pBV220B1 was constructed and transformed into E.coli DH5α.A 60 kD protein was induced by 42℃ and its expression was temperature\|dependent.After 6h induction,the target protein occupied 50% of the total protein.The expressed product existed in both inclusion body and soluble proteins in the cells.The amount of the soluble detoxifying enzyme increased along with the induction time.The data of detoxifying experiments indicated that the detoxifying enzyme in expression strain of E.coli can detoxified toxicity of organophosphate insecticides,it showed a clear detoxifying affect on hens poisoned by organophosphate insecticides.
The full\|length cDNA of mosquito esterase B1 had been isolated,and subcloned into pBV220.The recombinant vector pBV220B1 was constructed and transformed into E.coli DH5α.A 60 kD protein was induced by 42℃ and its expression was temperature\|dependent.After 6h induction,the target protein occupied 50% of the total protein.The expressed product existed in both inclusion body and soluble proteins in the cells.The amount of the soluble detoxifying enzyme increased along with the induction time.The data of detoxifying experiments indicated that the detoxifying enzyme in expression strain of E.coli can detoxified toxicity of organophosphate insecticides,it showed a clear detoxifying affect on hens poisoned by organophosphate insecticides.
出处
《生物工程学报》
CAS
CSCD
北大核心
2001年第2期199-202,共4页
Chinese Journal of Biotechnology
基金
国家"八六三"计划项目基金
国家自然科学基金! (批准号 :39980 0 34)资助
关键词
解毒酶基因
分子克隆
高效表达
解毒
昆虫
抗药性
杀虫剂抗性
Culex quinquefasciatus, esterase B1 gene cloning, high\|level expression, detoxification, E.coli
