摘要
The effects of the incubation temperature,initial pH of the medium,carbon source and nitrogen source on the production of Crypthecodinium cohnii C98 were studied.Through orthogonal experiments,the optimum culture medium was obtained (g/L):Glucose,65;Yeast extract,2.0;KNO 3 3.0,KH 2PO 4 1.0,MgSO 4·7H 2O 0.6,NaCl 5.8,CaCl 2·2H 2O 0.1,ZnSO 4·7H 2O 0.0067,FeCl 3·6H 2O 0.014,CuSO 4·5H 2O 0.0004,MnSO 4·H 2O 0.0002.Under the optimum culture conditions,the microalgae dry cell weight and eicosapentaenoic acid was 18.3g/L and 0.891 g/L,respectively.The submersion culture process was analysed.
The effects of the incubation temperature,initial pH of the medium,carbon source and nitrogen source on the production of Crypthecodinium cohnii C98 were studied.Through orthogonal experiments,the optimum culture medium was obtained (g/L):Glucose,65;Yeast extract,2.0;KNO 3 3.0,KH 2PO 4 1.0,MgSO 4·7H 2O 0.6,NaCl 5.8,CaCl 2·2H 2O 0.1,ZnSO 4·7H 2O 0.0067,FeCl 3·6H 2O 0.014,CuSO 4·5H 2O 0.0004,MnSO 4·H 2O 0.0002.Under the optimum culture conditions,the microalgae dry cell weight and eicosapentaenoic acid was 18.3g/L and 0.891 g/L,respectively.The submersion culture process was analysed.
出处
《生物工程学报》
CAS
CSCD
北大核心
2001年第2期221-223,共3页
Chinese Journal of Biotechnology
关键词
隐甲藻
深层培养
二十碳五烯酸
生产
Crypthecodinium cohnii, submersion culture,eicosapentaenoic acid
