小卷蛾线虫脱水休眠的形态与耗氧量

Morphology and oxygen consumption of Steinernema carpocapsae in anhydrobiosis

报道了小卷蛾线虫Steinernemacarpocapsae (BJ品系 )在高渗液中脱水进入休眠的形态变化和耗氧量。结果表明 :脱水线虫的形态变化与耗氧量相关 ,线虫在高渗液第 30h内 ,脱水程度越强 ,耗氧量越低。线虫脱水进入休眠从形态上分为螺旋、鞘壁分离和侧线弯曲三个阶段。在螺旋阶段脱水线虫代谢开始减慢 ,耗氧量较对照减少了 14%。在鞘壁分离阶段脱水线虫开始进入休眠 ,耗氧量较对照减少了 6 5% ,加水后线虫在 10min内 10 0 %复苏。在侧线弯曲阶段Ⅰ脱水线虫进入了深度休眠 ,耗氧量较对照减少了 79% ,加水后在 30min内 10 0 %复苏。

The relationship between morphological change and oxygen consumption of infective anhydrobotic juveniles of Steinernema carpocapsae (BJ strain) was studied in the laboratory. The results showed the nematodes treated in SB high osmotic solution presented different morphologies. The morphological changes included three stages: coil, sheath cuticle separation and curved lateral lines. Metabolism of the coil nematodes went down and their oxygen consumption decreased by 14%. Oxygen consumption of the nematodes in sheath cuticle separation stage decreased by 65% when they began to enter anhydrobiotic state. All of them revived in water for 10 minutes. Oxygen consumption of the nematodes showing curved lateral lines decreased by 79%, indicating they entered into futher anhydrobiotic state.These nematodes revived entirely after 30 minutes in water.